AMPK alpha 1 Antibody (1G4) - Azide and BSA Free

Novus Biologicals | Catalog # H00005562-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G4

Format

Azide and BSA Free
View all AMPK alpha 1 Primary Antibodies »

Product Specifications

Immunogen

PRKAA1 (AAH12622, 451 a.a. ~ 550 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNYRSCQRSDSDAEAQGKSSEVSLTSSVTSLDSSPVDLTPRPGSHTIEFFEMCANLIKILAQ

Specificity

PRKAA1 - protein kinase, AMP-activated, alpha 1 catalytic subunit

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for AMPK alpha 1 Antibody (1G4) - Azide and BSA Free

Western Blot: AMPK alpha 1 Antibody (1G4) [H00005562-M01]

Western Blot: AMPK alpha 1 Antibody (1G4) [H00005562-M01]

Western Blot: AMPK alpha 1 Antibody (1G4) [H00005562-M01] - PRKAA1 monoclonal antibody (M01), clone 1G4 Analysis of PRKAA1 expression in HepG2.
ELISA: AMPK alpha 1 Antibody (1G4) [H00005562-M01]

ELISA: AMPK alpha 1 Antibody (1G4) [H00005562-M01]

ELISA: AMPK alpha 1 Antibody (1G4) [H00005562-M01] - Detection limit for recombinant GST tagged PRKAA1 is approximately 0.3ng/ml as a capture antibody.

Applications for AMPK alpha 1 Antibody (1G4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AMPK alpha 1

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Long Name

AMP-activated Protein Kinase alpha 1

Alternate Names

PRKAA1

Entrez Gene IDs

5562 (Human)

Gene Symbol

PRKAA1

OMIM

602739 (Human)

UniProt

AAH12622 (Human)

Additional AMPK alpha 1 Products

Product Documents for AMPK alpha 1 Antibody (1G4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for AMPK alpha 1 Antibody (1G4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
Intracellular Kinases
Obesity

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