Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free

Novus Biologicals | Catalog # NB110-85467

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Chicken

Cited:

Human, Avian - Chicken

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 Clone # MM0020-1F29

Format

Azide and BSA Free
View all Angiopoietin-2 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant Angiopoietin-2

Reactivity Notes

Chicken data from customer review.

Specificity

No cross reactivity with human Angiopoietin-1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2

Scientific Data Images for Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free [NB110-85467]

Immunocytochemistry/ Immunofluorescence: Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free [NB110-85467]

Immunocytochemistry/Immunofluorescence: Angiopoietin-2 Antibody (MM0020-1F29) [NB110-85467] - Staining of frozen human skin sections using Angiopoietin 2 antibody at 1:25 dilution. Image provided via product review by verified customer.
Immunohistochemistry: Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free [NB110-85467]

Immunohistochemistry: Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free [NB110-85467]

Immunohistochemistry: Angiopoietin-2 Antibody (MM0020-1F29) [NB110-85467] - Ang-2 (green) was detected in human skin (nevus) using Ang2-Alexa Fluor 488 antibody (1:40) in PBS for 1 hour. Nuclei were stained with DAPI (blue). Tissue was fixed with acetone. Image from a verified customer review. Image using the Alexa Fluor 488 format of this antibody.
Immunohistochemistry: Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free [NB110-85467]

Immunohistochemistry: Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free [NB110-85467]

Immunohistochemistry: Angiopoietin-2 Antibody (MM0020-1F29) [NB110-85467] - Normal Human placenta tissue
Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free

Immunohistochemistry: Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free [NB110-85467] -

As compared to the primary tumors, related omental metastases showed stronger expression of Ang-2 (a and b) and Tie-2 (e and f). Primary tumors are shown on the left column and related metastases on the right. The vascular endothelial expression was strong in Ang-2 (black arrow in a). Tie-1 expression did not differ significantly between the primary and the distal metastatic tumors (c and d). Tie-1 was expressed in the tumor stroma (black arrow in d). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33151982), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free

Immunohistochemistry: Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free [NB110-85467] -

As compared to the primary tumors, related omental metastases showed stronger expression of Ang-2 (a and b) and Tie-2 (e and f). Primary tumors are shown on the left column and related metastases on the right. The vascular endothelial expression was strong in Ang-2 (black arrow in a). Tie-1 expression did not differ significantly between the primary and the distal metastatic tumors (c and d). Tie-1 was expressed in the tumor stroma (black arrow in d). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33151982), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:25

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:200

Western Blot

1:500-1:1000
Application Notes
By Western blot, this antibody detects a band at ~60-75 kDa under reducing conditions. Has been reported to work in Immunohistochemistry-Frozen (PMID 22302773) and Immunofluorescence in a customer review.

Reviewed Applications

Read 2 reviews rated 3 using NB110-85467 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-2

Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2/Tek. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin 1 binding to Tie 2/Tek causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Alternate Names

ANGPT2

Entrez Gene IDs

285 (Human)

Gene Symbol

ANGPT2

UniProt

O15123

Additional Angiopoietin-2 Products

Product Documents for Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free

Customer Reviews for Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free (2)

3 out of 5
2 Customer Ratings
5 Stars
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3 Stars
100%
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Name: Bryan Tinsley
    Application: Immunofluorescence
    Sample Tested:
    Species: Other
    Verified Customer | Posted 08/26/2014
    indirect immunofluorescence data with anti-Ang2 on chicken comb
    Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free NB110-85467
  • Name: Corey.
    Application: Immunohistochemistry-Frozen
    Sample Tested: Frozen human skin sections
    Species: Human
    Verified Customer | Posted 07/12/2013
    Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free NB110-85467

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Protocols

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FAQs for Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: How do you dissolve the lyophilized Ab? Water or PBS? And in what concentration?

    A: The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). 100 ug antibody is lyophilized from 40 ul of 1 x PBS. We suggest reconstituting the antibody with 500 ul sterile 1 x PBS (or to be perfect, with 40 ul H2O plus 460 ul PBS), to give a final concentration of 200 ug/ml. Alternatively, you may reconstitute the antibody in 40 ul H2O if a small volume is preferred.

  • Q: I have a question regarding your Angiopoietin 2 Antibody (MM0020-1F29). I am interested in differentiating Ang2 from mouse origin and Ang2 from human origin. Does this antibody cross react in any way with mouse Ang2?

    A: This particular product has only been tested against human samples. We have not yet tested for cross reactivity with other species. Based on the homology of the full length protein, I would suspect that there may be some cross reaction. I located the amino acid range for this product. It is between aa70-aa496 of recombinant human Angiopoietin-2 (O15123). If you would be interested in testing this novel species, please take a look at our Innovator's Reward program.

  • Q: How do you dissolve the lyophilized Ab? Water or PBS? And in what concentration?

    A: The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). 100 ug antibody is lyophilized from 40 ul of 1 x PBS. We suggest reconstituting the antibody with 500 ul sterile 1 x PBS (or to be perfect, with 40 ul H2O plus 460 ul PBS), to give a final concentration of 200 ug/ml. Alternatively, you may reconstitute the antibody in 40 ul H2O if a small volume is preferred.

  • Q: I have a question regarding your Angiopoietin 2 Antibody (MM0020-1F29). I am interested in differentiating Ang2 from mouse origin and Ang2 from human origin. Does this antibody cross react in any way with mouse Ang2?

    A: This particular product has only been tested against human samples. We have not yet tested for cross reactivity with other species. Based on the homology of the full length protein, I would suspect that there may be some cross reaction. I located the amino acid range for this product. It is between aa70-aa496 of recombinant human Angiopoietin-2 (O15123). If you would be interested in testing this novel species, please take a look at our Innovator's Reward program.

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