Annexin A2 Antibody (ANEX 3D5/4) - BSA Free

Novus Biologicals | Catalog # NB100-2724

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # ANEX 3D5/4

Format

BSA Free
View all Annexin A2 Primary Antibodies »

Product Specifications

Immunogen

Mixture of native membrane proteins from human bone tissues which would include whole native annexin proteins

Localization

Cell Membrane

Specificity

ANEX 3D5/4 detects native protein. Recommended concentration range: 1-10 ug/ml.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for Annexin A2 Antibody (ANEX 3D5/4) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): Endothelial cells. Use in Immunoassay reported in scientific literature (PMID: 31035430).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Annexin A2

The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin II is a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ANX2L4, ANXA2, CAL1H, LIP2, Lipocortin-2, LPC2D, PAP-IV

Entrez Gene IDs

302 (Human)

Gene Symbol

ANXA2

Additional Annexin A2 Products

Product Documents for Annexin A2 Antibody (ANEX 3D5/4) - BSA Free

Certificate of Analysis

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Product Specific Notices for Annexin A2 Antibody (ANEX 3D5/4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Annexin A2 Antibody (ANEX 3D5/4) - BSA Free

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Protocols

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FAQs

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