Annexin A3 Antibody (4F1) - Azide and BSA Free

Novus Biologicals | Catalog # H00000306-M12

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4F1

Format

Azide and BSA Free
View all Annexin A3 Primary Antibodies »

Product Specifications

Immunogen

ANXA3 (AAH00871, 1 a.a. ~ 323 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASIWVGHRGTVRDYPDFSPSVDAEAIQKAIRGIGTDEKMLISILTERSNAQRQLIVKEYQAAYGKELKDDLKGDLSGHFEHLMVALVTPPAVFDAKQLKKSMKGAGTNEDALIEILTTRTSRQMKDISQAYYTVYKKSLGDDISSETSGDFRKALLTLADGRRDESLKVDEHLAKQDAQILYKAGENRWGTDEDKFTEILCLRSFPQLKLTFDEYRNISQKDIVDSIKGELSGHFEDLLLAIVNCVRNTPAFLAERLHRALKGIGTDEFTLNRIMVSRSEIDLLDIRTEFKKHYGYSLYSAIKSDTSGDYEITLLKICGGDD

Specificity

ANXA3 (4F1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Annexin A3 Antibody (4F1) - Azide and BSA Free

Western Blot: Annexin A3 Antibody (4F1) [H00000306-M12]

Western Blot: Annexin A3 Antibody (4F1) [H00000306-M12]

Western Blot: Annexin A3 Antibody (4F1) [H00000306-M12] - Analysis of ANXA3 expression in transfected 293T cell line by ANXA3 monoclonal antibody (M12), clone 4F1. Lane 1: ANXA3 transfected lysatE (37.145 KDa). Lane 2: Non-transfected lysate.
Western Blot: Annexin A3 Antibody (4F1) [H00000306-M12]

Western Blot: Annexin A3 Antibody (4F1) [H00000306-M12]

Western Blot: Annexin A3 Antibody (4F1) [H00000306-M12] - Western blot analysis of ANXA3 over-expressed 293 cell line. Lane 2 cotransfected with ANXA3 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000306-M12. GAPDH (36.1 kDa) used as loading control.
ELISA: Annexin A3 Antibody (4F1) [H00000306-M12]

ELISA: Annexin A3 Antibody (4F1) [H00000306-M12]

ELISA: Annexin A3 Antibody (4F1) [H00000306-M12] - Detection limit for recombinant GST tagged ANXA3 is approximately 1ng/ml as a capture antibody.

Applications for Annexin A3 Antibody (4F1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Annexin A3

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation.

Alternate Names

ANXA3, Calcimedin 35-alpha, Lipocortin III, PAP-III

Entrez Gene IDs

306 (Human)

Gene Symbol

ANXA3

OMIM

106490 (Human)

UniProt

AAH00871 (Human)

Additional Annexin A3 Products

Product Documents for Annexin A3 Antibody (4F1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Annexin A3 Antibody (4F1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Annexin A3 Antibody (4F1) - Azide and BSA Free

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Protocols

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FAQs

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