Annexin A6 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-99727

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Annexin A6 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human Annexin A6 (Accession#: P08133; Met1-Asp673)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Annexin A6 Antibody - Azide and BSA Free

Western Blot: Annexin A6 Antibody [NBP2-99727]

Western Blot: Annexin A6 Antibody [NBP2-99727]

Western Blot: Annexin A6 Antibody [NBP2-99727] - Anti-Annexin A6 rabbit polyclonal antibody at 1:1000 dilution. Lane A: NIH-3T3 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: NCI-H1299 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 76 kDa. Observed band size: 65 kDa (We are unsure as to the identity of these extra bands).
Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP2-99727]

Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP2-99727]

Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP2-99727] - Immunochemical staining of human Annexin A6 in human testis with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP2-99727]

Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP2-99727]

Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP2-99727] - Immunochemical staining of human Annexin A6 in human liver with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP2-99727]

Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP2-99727]

Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP2-99727] - Immunochemical staining of human Annexin A6 in human pancreas with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
Immunoprecipitation: Annexin A6 Antibody [NBP2-99727]

Immunoprecipitation: Annexin A6 Antibody [NBP2-99727]

Immunoprecipitation: Annexin A6 Antibody [NBP2-99727] - Annexin A6 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg NIH-3T3 Whole Cell Lysate. Lane C: 0.5 mg 293T Whole Cell Lysate. 2 ul anti-Annexin A6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Annexin A6 rabbit polyclonal antibody, at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 76 kDa. Observed band size: 76 kDa

Applications for Annexin A6 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry

1:10000-1:20000

Immunohistochemistry-Paraffin

1:10000-1:20000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Annexin A6

The Annexins constitute a family of structurally-related, relatively abundant proteins that exhibit Ca2+-dependent binding to phospholipids. Annexins function in multiple aspects of cell biology including regulation of membrane trafficking, transmembrane channel activity, inhibition of phospholipase A2, inhibition of coagulation and mediation of cell-matrix interactions. Annexin A13 is considered the original progenitor of the 12 members of vertebrate Annexins. The expression of Annexin A13 is highly tissue-specific, being expressed only in intestinal and kidney epithelial cells. This expression is associated with a highly differentiated intracellular transport function. Two alternative splicing isoforms of Annexin A13 exist, both of which bind to rafts.

Alternate Names

ANX6, ANXA6, Calelectrin, CBP68, CPB-II, Lipocortin VI

Gene Symbol

ANXA6

Additional Annexin A6 Products

Product Documents for Annexin A6 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Annexin A6 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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