Annexin V Apoptosis Kit [FITC]

Novus Biologicals | Catalog # NBP2-29373

Novus Biologicals
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Key Product Details

Species

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Kit Type

Apoptosis Kit

Conjugate

FITC

Product Summary for Annexin V Apoptosis Kit [FITC]

Annexin V Apoptosis Kit [FITC] can identify apoptosis at an earlier stage than kits based on DNA fragmentation in the nucleus. However, it is like most assays and has limitations. Since Annexin V staining precedes the loss of membrane integrity which accompanies the later stages of cell death resulting from either apoptotic or necrotic processes, staining with Annexin V-FITC is typically used in conjunction with a live/dead dye such as propidium iodide (PI) or 7-Amino-Actinomycin (7-AAD) to allow the investigator to identify early apoptotic cells (PI negative, Annexin V-FITC positive) from dead cells (PI positive, AnnexinV-FITC positive). Viable cells with intact membranes exclude PI, whereas the membranes of dead and damaged cells are permeable to PI. For this reason, Annexin V staining has to be performed on live cells as opposed to assays which require para- formaldehyde/ethanol fixed cells.

The Annexin V assay works in the following manner: Cells that are viable are both Annexin V-FITC and PI negative. While cells that are in early apoptosis are Annexin V-FITC positive and PI negative and cells that are in late apoptosis or already dead are both FITC Annexin V and PI positive. This assay does not distinguish between cells that have undergone apoptotic death versus those that have died as a result of a necrotic pathway because the dead cells will stain with both Annexin V-FITC and PI (see image). However, when apoptosis is measured over time, cells can be often tracked from Annexin V- FITC and PI negative (viable, or no measurable apoptosis), to Annexin V-FITC positive and PI negative (early apoptosis, membrane integrity is present) and finally to Annexin V- FITC and PI positive (end stage apoptosis and death). The movement of cells through these three stages suggests apoptosis. In contrast, a single observation indicating that cells are both Annexin V-FITC and PI positive, in of itself, reveals less information about the process by which the cells underwent their demise. For this reason, it is a good idea to analyze samples from multiple time points.

Product Specifications

Application Notes

Use in Flow cell surface reported in scientific literature (PMID: 23955790). Use the Control Cells provided to set up compensation and quadrants. The Control Cells are positive for both Annexin V-FITC and PI.
The basal level of apoptosis and necrosis varies considerably within a give cell population. Even in the absence of induced apoptosis, most cell populations will contain a minor percentage of cells that are positive for apoptosis (Annexin V-FITC positive, PI negative) and dead, necrotic, or in the late stages of apoptosis (Annexin V-FITC positive, PI positive). Thus, an untreated cell population is used to define the basal level of apoptotic and dead cells.
Determine the percentage of cells that have been induced to undergo apoptosis by subtracting the percentage of apoptotic cells in the untreated from the treated population. Since cell death is the eventual outcome of cells undergoing apoptosis, cells in the late stages of apoptosis will have a damaged membrane and stain positive for PI as well as for Annexin V-FITC.
Consequently cells which have undergone necrosis are not distinguishable from those which have undergone apoptosis. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 25947082). Use in FLOW cytometry reported in scientific literature (PMID: 27448441).

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23955790). Mouse reactivity reported in scientific literature (PMID: 22566271). Rat reactivity reported in scientific literature (PMID: 25947082)

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Scientific Data Images for Annexin V Apoptosis Kit [FITC]

Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373]

Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373]

Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Adherent RAW cells treated with actinomycin-D, detached with a cell detachment solution and stained with the Annexin V Apoptosis Kit [FITC].
Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373]

Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373]

Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Controls run before the experimental samples are analyzed, to adjust the fluorescent compensation on the flow cytometer due to the FITC signal from the Annexin V-FITC bleeding into the Propidium Iodide signal.

Kit Contents for Annexin V Apoptosis Kit [FITC]

  • 10X Binding Buffer
  • 10X PBS Solution
  • Annexin V-FITC
  • Compensation Control Cells
  • Propidium Iodide (PI) Solution

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C. Do not freeze.

Background: Annexin V

Apoptosis is the term that describes programmed cell death. It is believed to take place in the majority of animal and plant cells. It is a distinct event that triggers characteristic morphological and biological changes in the cellular life cycle. One of the early events that happens in the apoptotic pathway is the loss of plasma membrane asymmetry. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, thereby exposing PS to the external cellular environment. Annexin V when labeled with a fluorescent tag, such as FITC, can be used with flow cytometry to measure this event. Annexin V is a 35-36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS, and binds to cells with exposed PS.

Alternate Names

ANX5, ANXA5, ANXV

Gene Symbol

ANXA5

Additional Annexin V Products

Product Documents for Annexin V Apoptosis Kit [FITC]

Certificate of Analysis

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Product Specific Notices for Annexin V Apoptosis Kit [FITC]

ADDITIONAL ITEMS REQUIRED (NOT INCLUDED IN THE KIT)
Distilled H2O
Flow Cytometer
Experimental Cells
Centrifuge
Positive Control Cell Line (recommended, not required)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Coagulation

Citations for Annexin V Apoptosis Kit [FITC]

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  • Annexin V Apoptosis Kit [FITC]
    Name: Anonymous
    Verified Customer | Posted 05/20/2021
    Annexin V Apoptosis Kit [FITC] NBP2-29373
  • Annexin V Apoptosis Kit [FITC]
    Name: Anonymous
    Verified Customer | Posted 02/20/2020
    Flow cytometry for Jurkat cells untreated (control) or treated with thapsigargin to promote cell death.
    Species: Human Sample type: Cell culture Assay time: 1-2 hours Followed instructions: Yes
    Annexin V Apoptosis Kit [FITC] NBP2-29373

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Protocols

View specific protocols for Annexin V Apoptosis Kit [FITC] (NBP2-29373):


Material Safety Data Sheet for Non Hazardous Products

Hazard Information
Chemical Name: Non hazardous products.
Chemical Formula: N/A
CAS Number: N/A
EEC-No: N/A

Hazard Identification
None

First Aid Measures
Eye Contact: None
Skin Contact: None
Inhalation: None
Ingestion: None

Accidental Release Measures
This product either does not contain hazardous constituents or the concentration of all chemical constituents are below the regulatory threshold limits described by Occupational Safety Health Administration Hazard Communication Standard 29 CFR 1910.1200 and the European Directive 91/155/EEC. 88/379/EEC, and 67/546/EEC.

Handling and Storage
Exposure Controls / Personal Protection
Other Precautions: None

Physical and Chemical Properties
Form: N/A
Color: N/A
Odor: N/A
Melting Point: N/A
Boiling Temperature: N/A
Density: N/A
Vapor Pressure: N/A
Solubility in Water: N/A
Flash Point: N/A
Explosion limits: N/A
Ignition Temperature: N/A

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FAQs for Annexin V Apoptosis Kit [FITC]

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    • Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?
      A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).

    • Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?
      A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.
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