Anti- Alemtuzumab (Anti-Idiotype) Antibody

R&D Systems | Catalog # MAB10246

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Multi-Species

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2499A
View all Alemtuzumab Primary Antibodies »

Product Specifications

Immunogen

Full-length Alemtuzumab

Specificity

Detects Alemtuzumab in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Anti- Alemtuzumab (Anti-Idiotype) Antibody

Detection of Alemtuzumab (Anti-CD52) on human PBMC lymphocytes antibody by Flow Cytometry.

Detection of Alemtuzumab (Anti-CD52) on human PBMC lymphocytes by flow cytometry.

Human PBMC lymphocytes were stained with Anti-Human CD52 (Research Grade Alemtuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9889), then Anti-Alemtuzumab (Anti-Idiotype) (Catalog # MAB10246, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by APC-conjugated anti-rabbit IgG secondary antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Applications for Anti- Alemtuzumab (Anti-Idiotype) Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMC lymphocytes stained with Anti-Human CD52 (Alemtuzumab), Catalog # MAB9889

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Alemtuzumab

The anti-Alemtuzumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Alemtuzumab. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Additional Alemtuzumab Products

Product Documents for Anti- Alemtuzumab (Anti-Idiotype) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Anti- Alemtuzumab (Anti-Idiotype) Antibody

For research use only

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FAQs for Anti- Alemtuzumab (Anti-Idiotype) Antibody

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  • Q: What is an anti-idiotype antibody?

    A: An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody.  We have anti-idiotype antibodies to commecially available therapeutic antibodies or  biosimilars.

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