Rabbit IgG Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF008
Detection  of Rabbit IgG Antibody by Simple WesternTM.
2 Images
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (2)

Rabbit IgG Biotinylated Antibody Summary

Species Reactivity
Rabbit
Specificity
Detects rabbit IgG heavy and light chains. In direct ELISAs,  less than 5% cross-reactivity with human IgG, mouse IgG and chicken IgY is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rabbit IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
Use at a 1:5000 dilution
 
Simple Western
Use at a 1:500 dilution
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Simple Western Detection  of Rabbit IgG Antibody by Simple WesternTM. View Larger

Detection of Rabbit IgG Antibody by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Survivin at approximately 24 kDa (as indicated) using 5 µg/mL (Lane A), 1.25 µg/mL (Lane B), or 0 µg/mL Lane C, negative control) of Rabbit Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody(Catalog # AF886) followed by 1:500 dilution of Biotin-conjugated Anti-Rabbit IgG Secondary Antibody (BAF008). Streptavidin-HRP was used for detection. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection  of Rabbit IgG Antibody by Simple WesternTM. View Larger

Detection of Rabbit IgG Antibody by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 71 kDa (as indicated) using 5 µg/mL (Lane A), 1.25 µg/mL (Lane B), or 0 µg/mL Lane C, negative control) of Rabbit Anti-Human/Mouse/Rat HSP70/HSPA1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1663) followed by 1:500 dilution of Biotin-conjugated Anti-Rabbit IgG Secondary Antibody (BAF008). Streptavidin-HRP was used for detection. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citations for Rabbit IgG Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. The expression of SIRT1 regulates the metastaticplasticity of chondrosarcoma cells by inducing epithelial-mesenchymal transition
    Authors: H Feng, J Wang, J Xu, C Xie, F Gao, Z Li
    Sci Rep, 2017;7(0):41203.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  2. Effect of hyperinsulinemia during hemodialysis on the insulin-like growth factor system and inflammatory biomarkers: a randomized open-label crossover study.
    Authors: Reinhard M, Frystyk J, Jespersen B, Bjerre M, Christiansen J, Flyvbjerg A, Ivarsen P
    BMC Nephrol, 2013;14(0):80.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  3. Human corneal epithelium-derived thymic stromal lymphopoietin links the innate and adaptive immune responses via TLRs and Th2 cytokines.
    Authors: Ma P, Bian F, Wang Z, Zheng X, Chotikavanich S, Pflugfelder SC, Li DQ
    Invest. Ophthalmol. Vis. Sci., 2009;50(6):2702-9.
    Species: Human
    Sample Types: Antibody
    Applications: IHC Frozen
  4. A novel role of the lumican core protein in bacterial lipopolysaccharide-induced innate immune response.
    Authors: Wu F, Vij N, Roberts L, Lopez-Briones S, Joyce S, Chakravarti S
    J. Biol. Chem., 2007;282(36):26409-17.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Development

FAQs

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Reviews for Rabbit IgG Biotinylated Antibody

Average Rating: 4 (Based on 2 Reviews)

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Goat Anti-Rabbit IgG Biotinylated Antibody
By Anonymous on 05/01/2018
Application: IHC Sample Tested: Cancer Cells Species: Rabbit

Goat Anti-Rabbit IgG Biotinylated Antibody
By Anonymous on 02/22/2016
Application: Immunoblot Sample Tested: Cell Culture Samples Species: Multi-Species