Key Product Details

Species Reactivity

Rabbit

Applications

Western Blot, Simple Western

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Rabbit IgG

Specificity

Detects rabbit IgG heavy and light chains. In direct ELISAs,  less than 5% cross-reactivity with human IgG, mouse IgG and chicken IgY is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rabbit IgG Biotinylated Antibody

Detection of Rabbit IgG Antibody by Simple WesternTM.

Detection of Rabbit IgG Antibody by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Survivin at approximately 24 kDa (as indicated) using 5 µg/mL (Lane A), 1.25 µg/mL (Lane B), or 0 µg/mL Lane C, negative control) of Rabbit Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF886) followed by 1:500 dilution of Biotin-conjugated Anti-Rabbit IgG Secondary Antibody (BAF008). Streptavidin-HRP was used for detection. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Rabbit IgG Antibody by Simple WesternTM.

Detection of Rabbit IgG Antibody by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 71 kDa (as indicated) using 5 µg/mL (Lane A), 1.25 µg/mL (Lane B), or 0 µg/mL Lane C, negative control) of Rabbit Anti-Human/Mouse/Rat HSP70/HSPA1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1663) followed by 1:500 dilution of Biotin-conjugated Anti-Rabbit IgG Secondary Antibody (BAF008). Streptavidin-HRP was used for detection. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Rabbit IgG Biotinylated Antibody

Application
Recommended Usage

Simple Western

Use at a 1:500 dilution

Western Blot

Use at a 1:5000 dilution

Reviewed Applications

Read 3 reviews rated 4.3 using BAF008 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Rabbit IgG Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rabbit IgG Biotinylated Antibody

For research use only

Citations for Rabbit IgG Biotinylated Antibody

Customer Reviews for Rabbit IgG Biotinylated Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 3 of 3 reviews Showing All
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  • Rabbit IgG Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/08/2018
  • Goat Anti-Rabbit IgG Biotinylated Antibody
    Name: Danlee Enzler
    Application: Immunohistochemistry
    Sample Tested: Cancer Cells
    Species: Rabbit
    Verified Customer | Posted 05/01/2018
  • Goat Anti-Rabbit IgG Biotinylated Antibody
    Name: Anonymous
    Application: Immunoblot
    Sample Tested: Cell Culture Samples
    Species: Multi-Species
    Verified Customer | Posted 02/22/2016

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FAQs

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