Key Product Details

Validated by

Biological Validation

Species Reactivity

Rabbit

Applications

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Goat IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Rabbit IgG

Specificity

Rabbit IgG specific IgG was purified by rabbit IgG affinity chromatography.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rabbit IgG Fluorescein‑conjugated Antibody

Detection of Rabbit IgG Primary Antibody by Flow Cytometry

Detection of Rabbit IgG Primary Antibody by Flow Cytometry

Daudi human Burkitt's lymphoma cell line untreated (open histogram) or treated (filled histogram) was stained with Rabbit Anti-Human Phospho-STAT2 (Y689) Monoclonal Antibody (Catalog # MAB2890), followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol.

Applications for Rabbit IgG Fluorescein‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rabbit origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Rabbit IgG Fluorescein‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rabbit IgG Fluorescein‑conjugated Antibody

For research use only

Citations for Rabbit IgG Fluorescein‑conjugated Antibody

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FAQs

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