Rabbit IgG Fluorescein-conjugated Antibody

(5 citations)   
  • Species Reactivity
    Rabbit
  • Specificity
    Rabbit IgG specific IgG was purified by rabbit IgG affinity chromatography.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Rabbit IgG
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rabbit origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of RabbitIgG Primary Antibody by Flow Cytometry Daudi human Burkitt's lymphoma cell line untreated (open histogram) or treated (filled histogram) was stained with Rabbit Anti-Human Phospho-STAT2 (Y689) Monoclonal Antibody (Catalog #MAB2890), followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #FC004) and permeabilized with 90% methanol.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. CD147 promotes the proliferation, invasiveness, migration and angiogenesis of human lung carcinoma cells
    Authors: S Yang, F Qi, C Tang, H Wang, H Qin, X Li, J Li, W Wang, C Zhao, H Gao
    Oncol Lett, 2017;13(2):898-904.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Tacrolimus Protects Podocytes from Injury in Lupus Nephritis Partly by Stabilizing the Cytoskeleton and Inhibiting Podocyte Apoptosis.
    Authors: Liao R, Liu Q, Zheng Z, Fan J, Peng W, Kong Q, He H, Yang S, Chen W, Tang X, Yu X
    PLoS ONE, 2015;10(7):e0132724.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Complement c5a receptor facilitates cancer metastasis by altering T-cell responses in the metastatic niche.
    Authors: Vadrevu S, Chintala N, Sharma S, Sharma P, Cleveland C, Riediger L, Manne S, Fairlie D, Gorczyca W, Almanza O, Karbowniczek M, Markiewski M
    , 2014;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. The novel receptor tyrosine kinase Axl is constitutively active in B-cell chronic lymphocytic leukemia and acts as a docking site of nonreceptor kinases: implications for therapy.
    Authors: Ghosh AK, Secreto C, Boysen J, Sassoon T, Shanafelt TD, Mukhopadhyay D, Kay NE
    Blood, 2011;117(6):1928-37.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Leptin receptor expression and signaling in lymphocytes: kinetics during lymphocyte activation, role in lymphocyte survival, and response to high fat diet in mice.
    Authors: Papathanassoglou E, El-Haschimi K, Li XC, Matarese G, Strom T, Mantzoros C
    J. Immunol., 2006;176(12):7745-52.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Fluorescein-conjugated Control

Ctrl IC108F 1
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

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Goat Anti-Rabbit IgG Biotinylated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rabbit IgG Antibody

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Rabbit IgG VisUCyte HRP Polymer Antibody

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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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