Anti- Solanezumab (Anti-Idiotype) Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Solanezumab by Western Blot. Western blot shows Solanexumab, Trastuzumab (negative control), and Adalibumab (negative control). PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Anti- Solanezumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB10124) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Solanezumab at approximately 240 kDa (as indicated). Human IgG is shown as a loading control. This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of Anti-APP (Solanezumab) on Human PBMC by flow cytometry. Human PBMC were stained with Human anti-Human APP (Solanezumab) Monoclonal Antibody (Catalog # MAB9919), then either (A) Rabbit Anti-Solanezumab (Anti-Idiotype) (Catalog # MAB10124) or (B) normal rabbit IgG (Catalog # MAB1050) followed by APC-conjugated anti-rabbit IgG secondary antibody (Catalog # F0111) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The anti-Solanezumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Solanezumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.
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