Anti- Teprotumumab (Anti-Idiotype) Antibody

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Detection of Teprotumumab by Western Blot.
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Product Details
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Anti- Teprotumumab (Anti-Idiotype) Antibody Summary

Detects Teprotumumab in Western blots and direct ELISAs. No cross-reactivity with Cetuximab, Brentuximab, Aducanumab, and Ipilimumab is observed.
Recombinant Monoclonal Rabbit IgG Clone # 2373A
Protein A or G purified from cell culture supernatant
Full-length Teprotumumab
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Teprotumumab antibody by Western Blot. View Larger

Detection of Teprotumumab by Western Blot. Western blot shows Centuximab (negative control), Brentuximab (negative control), Teprotumumab, Aducanumab (negative control) and Ipilimumab (negative control) biosimilars. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Anti- Teprotumumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB96342) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Teprotumumab at approximately 240 kDa (as indicated). Human IgG is shown as a loading control. This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Teprotumumab

The anti-Teprotumumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Teprotumumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

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  1. What is an anti-idiotype antibody?

    • An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody.  We have anti-idiotype antibodies to commecially available therapeutic antibodies or  biosimilars.

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