AP2 alpha Antibody (3B5)

Novus Biologicals | Catalog # NB100-74359

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Chicken

Cited:

Mouse, Avian - Chicken

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 3B5
View all AP2 alpha Primary Antibodies »

Product Specifications

Immunogen

N-terminus of human AP2a.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

AP-2 (3B5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AP2 alpha Antibody (3B5)

Western Blot: AP2 alpha Antibody (3B5) [NB100-74359]

Western Blot: AP2 alpha Antibody (3B5) [NB100-74359]

Western Blot: AP2 alpha Antibody (3B5) [NB100-74359] - Analysis was performed on whole cell extracts (30 ug lysate) of PC-3 (Lane1), A-431 (Lane2), Mouse Placenta (Lane3) and RAW 264.7 (Lane5).
Immunocytochemistry/ Immunofluorescence: AP2 alpha Antibody (3B5) [NB100-74359]

Immunocytochemistry/ Immunofluorescence: AP2 alpha Antibody (3B5) [NB100-74359]

Immunocytochemistry/Immunofluorescence: AP2 alpha Antibody (3B5) [NB100-74359] - Analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) shows staining in Hela Cells. AP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing AP2 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Immunohistochemistry-Frozen: AP2 alpha Antibody (3B5) [NB100-74359]

Immunohistochemistry-Frozen: AP2 alpha Antibody (3B5) [NB100-74359]

Immunohistochemistry-Frozen: AP2 alpha Antibody (3B5) [NB100-74359] - AP2alpha staining on an E9.5 mouse pharyngeal mesoderm region. IHC-Fr image submitted by a verified customer review.
Western Blot: AP2 alpha Antibody (3B5) [NB100-74359]

Western Blot: AP2 alpha Antibody (3B5) [NB100-74359]

Western Blot: AP2 alpha Antibody (3B5) [NB100-74359] - Figure 1 illustrates Western blot detection of AP-2 from MCF7 cell lysate.
Immunocytochemistry/ Immunofluorescence: AP2 alpha Antibody (3B5) [NB100-74359]

Immunocytochemistry/ Immunofluorescence: AP2 alpha Antibody (3B5) [NB100-74359]

Immunocytochemistry/Immunofluorescence: AP2 alpha Antibody (3B5) [NB100-74359] - Analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) shows staining in U251 Cells. AP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Immunocytochemistry/ Immunofluorescence: AP2 alpha Antibody (3B5) [NB100-74359]

Immunocytochemistry/ Immunofluorescence: AP2 alpha Antibody (3B5) [NB100-74359]

Immunocytochemistry/Immunofluorescence: AP2 alpha Antibody (3B5) [NB100-74359] - Analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) shows staining in MCF-7 Cells. AP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Immunohistochemistry-Paraffin: AP2 alpha Antibody (3B5) [NB100-74359]

Immunohistochemistry-Paraffin: AP2 alpha Antibody (3B5) [NB100-74359]

Immunohistochemistry-Paraffin: AP2 alpha Antibody (3B5) [NB100-74359] - Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue.

Applications for AP2 alpha Antibody (3B5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:500

Immunohistochemistry

1:50

Immunohistochemistry-Frozen

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

1:100 - 1:250

Western Blot

1:50 - 1:200
Application Notes
In WB: Detects a 45 - 50 kDa protein representing AP-2. Block with BSA only (not milk). IHC-Fr usage was reported in scientific literature (PMID: 24996922).

Reviewed Applications

Read 1 review rated 5 using NB100-74359 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AP2 alpha

Activation Protein transcription factor 2 (AP2) is a developmentally regulated transcription factor that recognizes a specific sequence CCCCAGGC or related sequences present in several promoter and enhancer gene regions including the promoters of the AP2 genes themselves. There are three different but highly homologous AP2 isoforms: alpha, beta, and gamma (ERF-1), encoded by three different retinoic acid-responsive genes. Each form of AP2 possesses the DNA binding domain (basic helix-span-helix motif) characteristic of this family of transcription factors. The AP2 proteins are normally expressed in ectodermally derived vertebrate tissues where they are necessary for normal growth and development. The factors have also been implicated in the control of cell proliferation, viral transformation, and oncogenesis. AP2 seems to play in important role in human breast cancer.

Alternate Names

Activating enhancer-binding protein 2-alpha, Activator protein 2, AP-2 transcription factor, AP2-alpha, AP2TFAP-2alpha, AP-2transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha), BOFS, FLJ51761, TFAP2, transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha), transcription factor AP-2-alpha

Entrez Gene IDs

7020 (Human); 21418 (Mouse)

Gene Symbol

TFAP2A

UniProt

P05549 (Human)

Additional AP2 alpha Products

Product Documents for AP2 alpha Antibody (3B5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for AP2 alpha Antibody (3B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for AP2 alpha Antibody (3B5)

Customer Reviews for AP2 alpha Antibody (3B5) (1)

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Customer Images

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  • AP2 alpha Antibody (3B5)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: E9.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 06/23/2021
    AP2alpha staining on an E9.5 mouse pharyngeal mesoderm region.
    Fixed 4% PFA overnight. Blocked with 1% BSA Primary antibody dilution - 20ug/mL Secondary antibody - Invitrogen Alexa Fluor 488 Secondary antibody dilution - 1:1000 Stained on a E9.5 mouse pharyngeal mesoderm region
    AP2 alpha Antibody (3B5) NB100-74359

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Protocols

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