AP2M1 Antibody (OTI1E9)

Novus Biologicals | Catalog # NBP2-00834

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1E9
View all AP2M1 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 97-383 of human AP2M1(NP_001020376) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

49.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AP2M1 Antibody (OTI1E9)

Western Blot: AP2M1 Antibody (1E9) [NBP2-00834] Analysis of extracts (35ug) from 9 different cell lines by using anti-AP2M1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: AP2M1 Antibody (OTI1E9) [NBP2-00834]

Immunocytochemistry/ Immunofluorescence: AP2M1 Antibody (OTI1E9) [NBP2-00834]

Immunocytochemistry/Immunofluorescence: AP2M1 Antibody (1E9) [NBP2-00834] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY AP2M1.
Immunohistochemistry-Paraffin: AP2M1 Antibody (OTI1E9) [NBP2-00834]

Immunohistochemistry-Paraffin: AP2M1 Antibody (OTI1E9) [NBP2-00834]

Immunohistochemistry-Paraffin: AP2M1 Antibody (1E9) [NBP2-00834] - Staining of paraffin-embedded Human colon tissue using anti-AP2M1 mouse monoclonal antibody.
Western Blot: AP2M1 Antibody (OTI1E9) [NBP2-00834]

Western Blot: AP2M1 Antibody (OTI1E9) [NBP2-00834]

Western Blot: AP2M1 Antibody (1E9) [NBP2-00834] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AP2M1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AP2M1.

Applications for AP2M1 Antibody (OTI1E9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200-500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.65 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AP2M1

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Alternate Names

Adaptin-mu2, adaptor-related protein complex 2, mu 1 subunit, AP-2 mu chain, Clathrin assembly protein complex 2 medium chain, clathrin coat adaptor protein AP50, Clathrin coat assembly protein AP50, Clathrin coat-associated protein AP50, clathrin-associated/assembly/adaptor protein, medium 1, HA2 50 kDa subunit, KIAA0109, mu subunit, Plasma membrane adaptor AP-2 50 kDa protein, plasma membrane adaptor AP-2 50kDA protein

Entrez Gene IDs

1173 (Human)

Gene Symbol

AP2M1

Additional AP2M1 Products

Product Documents for AP2M1 Antibody (OTI1E9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for AP2M1 Antibody (OTI1E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for AP2M1 Antibody (OTI1E9)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for AP2M1 Antibody (OTI1E9)

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  • Q: I have a question about your product,named AP2M1 antibody (1E9) NBP2-00834. I didn't find the concentration of this anbody on the product page, could you please tell me the concentrarion infomation of this antibody?

    A: Current lot of AP2M1 antibody NBP2-00834 is supplied at 0.65 mg/ml. The vial is 100ul.

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