APAF-1 Antibody (2H9A1) - BSA Free

Novus Biologicals | Catalog # NBP2-61868

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 2H9A1

Format

BSA Free
View all APAF-1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human APAF-1 (AA: 1138-1237) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

141.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for APAF-1 Antibody (2H9A1) - BSA Free

Western Blot: APAF-1 Antibody (2H9A1)BSA Free [NBP2-61868]

Western Blot: APAF-1 Antibody (2H9A1)BSA Free [NBP2-61868]

Western Blot: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis using APAF1 mAb against human APAF1 (AA: 1138-1237) recombinant protein. (Expected MW is 37 kDa)
Immunocytochemistry/ Immunofluorescence: APAF-1 Antibody (2H9A1) - BSA Free [NBP2-61868]

Immunocytochemistry/ Immunofluorescence: APAF-1 Antibody (2H9A1) - BSA Free [NBP2-61868]

Immunocytochemistry/Immunofluorescence: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis of Hela cells using APAF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Flow Cytometry: APAF-1 Antibody (2H9A1) - BSA Free [NBP2-61868]

Flow Cytometry: APAF-1 Antibody (2H9A1) - BSA Free [NBP2-61868]

Flow Cytometry: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis of Hela cells using APAF1 mouse mAb (green) and negative control (red).
Western Blot: APAF-1 Antibody (2H9A1)BSA Free [NBP2-61868]

Western Blot: APAF-1 Antibody (2H9A1)BSA Free [NBP2-61868]

Western Blot: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis using APAF1 mAb against HEK293 (1) and APAF1 (AA: 1138-1237)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: APAF-1 Antibody (2H9A1) - BSA Free [NBP2-61868]

ELISA: APAF-1 Antibody (2H9A1) - BSA Free [NBP2-61868]

ELISA: APAF-1 Antibody (2H9A1) [NBP2-61868] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for APAF-1 Antibody (2H9A1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APAF-1

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

Long Name

Apoptotic Peptidase Activating Factor 1

Alternate Names

APAF1

Gene Symbol

APAF1

Additional APAF-1 Products

Product Documents for APAF-1 Antibody (2H9A1) - BSA Free

Certificate of Analysis

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Product Specific Notices for APAF-1 Antibody (2H9A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail