APE Antibody (CPTC-APEX1-2) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-08370
![Western Blot: APE Antibody (CPTC-APEX1-2)Azide and BSA Free [NBP3-08370]](https://resources.rndsystems.com/images/products/APE-Antibody-CPTC-APEX1-2-Western-Blot-NBP3-08370-img0005.jpg "Western Blot: APE Antibody (CPTC-APEX1-2)Azide and BSA Free [NBP3-08370]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # CPTC-APEX1-2
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant human full-length APE protein (Uniprot: P27695)
Localization
Mitochondrion, Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07864).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for APE Antibody (CPTC-APEX1-2) - Azide and BSA Free
Western Blot: APE Antibody (CPTC-APEX1-2)Azide and BSA Free [NBP3-08370]
Western Blot: APE Antibody (CPTC-APEX1-2) [NBP3-08370] - Western Blot Analysis of Human A431, A549, PC3, HAP1, HePG2, cell lysate using APE Mouse Monoclonal Antibody (CPTC-APEX1-2).![Immunocytochemistry/ Immunofluorescence: APE Antibody (CPTC-APEX1-2) - Azide and BSA Free [NBP3-08370]](https://resources.rndsystems.com/images/products/APE-Antibody-CPTC-APEX1-2-Immunocytochemistry-Immunofluorescence-NBP3-08370-img0002.jpg "Immunocytochemistry/ Immunofluorescence: APE Antibody (CPTC-APEX1-2) - Azide and BSA Free [NBP3-08370]")
Immunocytochemistry/ Immunofluorescence: APE Antibody (CPTC-APEX1-2) - Azide and BSA Free [NBP3-08370]
Immunocytochemistry/Immunofluorescence: APE Antibody (CPTC-APEX1-2) [NBP3-08370] - Immunofluorescence Analysis of human HeLa cells labeling APE with APE Mouse Monoclonal Antibody (CPTC-APEX1-2) followed by goat anti- Mouse IgG-CF488 (Green). Phalloidin CF640 stains the membrane red.![Immunohistochemistry-Paraffin: APE Antibody (CPTC-APEX1-2) - Azide and BSA Free [NBP3-08370]](https://resources.rndsystems.com/images/products/APE-Antibody-CPTC-APEX1-2-Immunohistochemistry-Paraffin-NBP3-08370-img0004.jpg "Immunohistochemistry-Paraffin: APE Antibody (CPTC-APEX1-2) - Azide and BSA Free [NBP3-08370]")
Immunohistochemistry-Paraffin: APE Antibody (CPTC-APEX1-2) - Azide and BSA Free [NBP3-08370]
Immunohistochemistry-Paraffin: APE Antibody (CPTC-APEX1-2) [NBP3-08370] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with APE Mouse Monoclonal Antibody (CPTC-APEX1-2).![Flow Cytometry: APE Antibody (CPTC-APEX1-2) - Azide and BSA Free [NBP3-08370]](https://resources.rndsystems.com/images/products/APE-Antibody-CPTC-APEX1-2-Flow-Cytometry-NBP3-08370-img0001.jpg "Flow Cytometry: APE Antibody (CPTC-APEX1-2) - Azide and BSA Free [NBP3-08370]")
Flow Cytometry: APE Antibody (CPTC-APEX1-2) - Azide and BSA Free [NBP3-08370]
Flow Cytometry: APE Antibody (CPTC-APEX1-2) [NBP3-08370] - Flow Cytometric Analysis of HeLa cells using APE Mouse Monoclonal Antibody (CPTC-APEX1-2). Goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).![Immunohistochemistry-Paraffin: APE Antibody (CPTC-APEX1-2) - Azide and BSA Free [NBP3-08370]](https://resources.rndsystems.com/images/products/APE-Antibody-CPTC-APEX1-2-Immunohistochemistry-Paraffin-NBP3-08370-img0003.jpg "Immunohistochemistry-Paraffin: APE Antibody (CPTC-APEX1-2) - Azide and BSA Free [NBP3-08370]")
Immunohistochemistry-Paraffin: APE Antibody (CPTC-APEX1-2) - Azide and BSA Free [NBP3-08370]
Immunohistochemistry-Paraffin: APE Antibody (CPTC-APEX1-2) [NBP3-08370] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with APE Mouse Monoclonal Antibody (CPTC-APEX1-2).![Protein Array: APE Antibody (CPTC-APEX1-2) - Azide and BSA Free [NBP3-08370]](https://resources.rndsystems.com/images/products/APE-Antibody-CPTC-APEX1-2-Protein-Array-NBP3-08370-img0006.jpg "Protein Array: APE Antibody (CPTC-APEX1-2) - Azide and BSA Free [NBP3-08370]")
Protein Array: APE Antibody (CPTC-APEX1-2) - Azide and BSA Free [NBP3-08370]
Protein Array: APE Antibody (CPTC-APEX1-2) [NBP3-08370] - Analysis of Protein Array containing more than 19,000 full-length human proteins using APE Mouse Monoclonal Antibody (CPTC-APEX1-2).Applications for APE Antibody (CPTC-APEX1-2) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: APE
For DNA repair, APE1 functions as a mammalian apurinic/apyrimidinic (AP) endodeoxyribonuclease responsible for the repair of AP sites in DNA lesions induced by oxidative and alkylating agents.
APEX1 also exerts a reversible nuclear redox activity to regulate the DNA binding affinity and the activity of transcriptional factors by regulating the redox status of their DNA-binding domains.
APE1 antibodies are useful for studies on both DNA repair and transcriptional regulation.
Long Name
Apurinic/Apyrimidinic Endonuclease
Alternate Names
APEX1
Gene Symbol
APEX1
Additional APE Products
Product Documents for APE Antibody (CPTC-APEX1-2) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for APE Antibody (CPTC-APEX1-2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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