APE Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-54653

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all APE Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a Recombinant Protein corresponding to amino acids: KLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGDEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLAD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit APE Antibody - BSA Free (NBP2-54653) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for APE Antibody - BSA Free

Western Blot: APE Antibody [NBP2-54653]

Western Blot: APE Antibody [NBP2-54653]

Western Blot: APE Antibody [NBP2-54653] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp Lane 4: Human cell line A-431 Lane 5: Human liver tissue Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: APE Antibody [NBP2-54653]

Immunocytochemistry/ Immunofluorescence: APE Antibody [NBP2-54653]

Immunocytochemistry/Immunofluorescence: APE Antibody [NBP2-54653] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.
Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653]

Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653]

Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653] - Staining of human skeletal muscle shows low expression as expected.
Western Blot: APE Antibody [NBP2-54653]

Western Blot: APE Antibody [NBP2-54653]

Western Blot: APE Antibody [NBP2-54653] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653]

Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653]

Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653] - Staining of human small intestine shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653]

Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653]

Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653] - Staining of human endometrium shows high expression.
Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653]

Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653]

Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653] - Staining in human endometrium and skeletal muscle tissues using anti-APEX1 antibody. Corresponding APEX1 RNA-seq data are presented for the same tissues.

Applications for APE Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APE

DNA-(apurinic or apyrimidinic site) lyase, also known as APE1 or APEX1, is a multifunctional mammalian proteins responsible for the cellular response to oxidative stress. Specifically, APE-1 both functions in the DNA base excision repair pathway and regulates the redox of transcriptional factors.

For DNA repair, APE1 functions as a mammalian apurinic/apyrimidinic (AP) endodeoxyribonuclease responsible for the repair of AP sites in DNA lesions induced by oxidative and alkylating agents.

APEX1 also exerts a reversible nuclear redox activity to regulate the DNA binding affinity and the activity of transcriptional factors by regulating the redox status of their DNA-binding domains.

APE1 antibodies are useful for studies on both DNA repair and transcriptional regulation.

Long Name

Apurinic/Apyrimidinic Endonuclease

Alternate Names

APEX1

Gene Symbol

APEX1

Additional APE Products

Product Documents for APE Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for APE Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Base Excision Repair

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Protocols

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FAQs

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