Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 2083D

Format

Azide and BSA Free
View all Apolipoprotein A-I/ApoA1 Primary Antibodies »

Product Specifications

Immunogen

rhApoA1 aa25-267

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

>90% by SDS PAGE

Scientific Data Images for Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free

Western Blot: Apolipoprotein A-I/ApoA1 Antibody (2083D)Azide and BSA Free [MAB3664]

Western Blot: Apolipoprotein A-I/ApoA1 Antibody (2083D)Azide and BSA Free [MAB3664]

Western Blot: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - Analysis of HepG2 cell lysate with 0.1 ug/ml of hApoA1 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hApoA1 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry: Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free [MAB3664]

Immunocytochemistry: Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free [MAB3664]

Immunocytochemistry: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - ApoA1 was detected in immersion fixed HepG2 cell using ApoA1 Monoclonal Antibody at 1 ug/ml dilution. Cells were stained using the NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.
Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free [MAB3664]

Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free [MAB3664]

Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - ApoA1 was detected in immersion fixed paraffin-embedded sections of human lung cancer using ApoA1 Monoclonal Antibody at 1 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells.
Flow Cytometry: Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free [MAB3664]

Flow Cytometry: Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free [MAB3664]

Flow Cytometry: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - An intracellular stain was performed on HepG2 cells with Apolipoprotein A-I/ApoA1 Antibody (2083D) MAB3664AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free [MAB3664]

Flow Cytometry: Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free [MAB3664]

Flow Cytometry: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Apolipoprotein A-I/ApoA1 Monoclonal Antibody (Catalog # MAB3664, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Applications for Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in the dark. Avoid freeze-thaw cycles.

Background: Apolipoprotein A-I/ApoA1

Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Gene Symbol

APOA1

Additional Apolipoprotein A-I/ApoA1 Products

Product Documents for Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Obesity

Customer Reviews for Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free and earn rewards!

Have you used Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies