Apolipoprotein L1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89033

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Apolipoprotein L1/APOL1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SNFLSLAGNTYQLTRGIGKDIRALRRARANLQSVPHASASRPRVTEPISAESGEQVERVNEPSILEMSRGVKLTDVAPVSFFLVLDVVYLVYESKHLHEGAKSETAEELKKVAQELEEKLNILNN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Apolipoprotein L1 Antibody - BSA Free

Western Blot: Apolipoprotein L1 Antibody [NBP1-89033]

Western Blot: Apolipoprotein L1 Antibody [NBP1-89033]

Western Blot: Apolipoprotein L1 Antibody [NBP1-89033] - Analysis in human cell lines A-431 and MCF-7 using anti-APOL1 antibody. Corresponding APOL1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Apolipoprotein L1 Antibody - BSA Free

Apolipoprotein L1 Antibody [NBP1-89033] -

Staining of human colon shows moderate to strong positivity in plasma in blood vessels.
Apolipoprotein L1 Antibody - BSA Free

Apolipoprotein L1 Antibody [NBP1-89033] -

Staining of human skin shows strong positivity in plasma in blood vessels.
Apolipoprotein L1 Antibody - BSA Free

Apolipoprotein L1 Antibody [NBP1-89033] -

Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Simple Western: Apolipoprotein L1 Antibody [NBP1-89033]

Simple Western: Apolipoprotein L1 Antibody [NBP1-89033]

Simple Western: Apolipoprotein L1 Antibody [NBP1-89033] - Simple Western lane view shows a specific band for APOL1 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: Apolipoprotein L1 Antibody [NBP1-89033]

Simple Western: Apolipoprotein L1 Antibody [NBP1-89033]

Simple Western: Apolipoprotein L1 Antibody [NBP1-89033] - Electropherogram image(s) of corresponding Simple Western lane view. Apolipoprotein L1 antibody was used at 1:30 dilution on RT-4 and U-251MG sp lysates(s).
Apolipoprotein L1 Antibody - BSA Free

Apolipoprotein L1 Antibody [NBP1-89033] -

Staining of human testis shows negative cytoplasmic positivity in cells in seminiferous ducts as expected.

Applications for Apolipoprotein L1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2500 -1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Simple Western

1:30

Western Blot

0.04 - 0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:30, apparent MW was 59 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apolipoprotein L1/APOL1

Apolipoproteins are protein components of plasma lipoproteins. The apolipoprotein L gene family encodes six highly homologous proteins designated apoL-I to -VI, which are associated with large high density type lipoproteins (HDL). The human apoL family maps to chromosome 22q12.1- 13.1 within a 127,000-bp region. apoL has been characterized as a pancreas specific, 383-amino acid, 42 kDa protein that contains a 12-amino acid secretory signal peptide. The apoL genes have TATA-less promoters and contain putative sterol regulatory elements, suggesting that transcription of these genes may be coordinated with that of the low density lipoprotein receptor and genes in pathways involving the synthesis of triglycerides and cholesterol. apoL homologs can undergo 10 fold changes in expression during atherosclerotic changes in vascular endothelial cells, which includes the inflammatory reaction of atherosclerotic lesions.

Long Name

Apolipoprotein L1

Alternate Names

APO-L, APO-L1, APO-LI, APOL, APOL1, FSGS4

Gene Symbol

APOL1

Additional Apolipoprotein L1/APOL1 Products

Product Documents for Apolipoprotein L1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Apolipoprotein L1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Obesity

Citations for Apolipoprotein L1 Antibody - BSA Free

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Protocols

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