APRIN Antibody

Novus Biologicals | Catalog # NB100-755

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1400 and the C-terminus (residue 1447) of human Precocious Dissociation of Sisters-5B using the numbering given in entry CAB69911.1 (GeneID 23047).

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for APRIN Antibody

Western Blot: APRIN Antibody [NB100-755]

Western Blot: APRIN Antibody [NB100-755]

Western Blot: APRIN Antibody [NB100-755] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiPds5b antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds
Immunohistochemistry-Paraffin: APRIN Antibody [NB100-755]

Immunohistochemistry-Paraffin: APRIN Antibody [NB100-755]

Immunohistochemistry-Paraffin: APRIN Antibody [NB100-755] - Section of human lung small cell carcinoma. Antibody: Affinity purified rabbit anti- Pds5B used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunoprecipitation: APRIN Antibody [NB100-755]

Immunoprecipitation: APRIN Antibody [NB100-755]

Immunoprecipitation: APRIN Antibody [NB100-755] - Detection of human Pds5b by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pds5b antibody NB100-755 used for IP at 3 ug per reaction. Pds5b was also immunoprecipitated by another rabbit anti-Pds5b antibody. For blotting immunoprecipitated Pds5b, NB100-755 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time time of 75 seconds.

Applications for APRIN Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: APRIN

Pds5B is a cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. It plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

Alternate Names

Androgen-induced proliferation inhibitorKIAA0979androgen induced inhibitor of proliferation, Androgen-induced prostate proliferative shutoff-associated protein AS3, androgen-induced shutoff 3, AS3APRIN, CG008, FLJ23236, PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae), sister chromatid cohesion protein PDS5 homolog B

Entrez Gene IDs

23047 (Human); 100710 (Mouse)

Gene Symbol

PDS5B

UniProt

Q9NTI5 (Human)

Additional APRIN Products

Product Documents for APRIN Antibody

Certificate of Analysis

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Product Specific Notices for APRIN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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