Aquaporin-2 [p Ser261] Antibody - Azide Free

Novus Biologicals | Catalog # NB100-61100

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Human, Canine

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Dot Blot

Cited:

Western Blot, Cytometric Bead Assay Standard, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all Aquaporin-2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser261 conjugated to KLH. Accession # P34080

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25977473). Canine reactivity reported in scientific literature (PMID: 22778181).

Modification

p Ser261

Specificity

Specific for endogenous levels of the ~29 kDa Aquaporin-2 protein phosphorylated at Ser261. Also recognizes the glycosylated form of AQP2 at ~37 kDa. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aquaporin-2 [p Ser261] Antibody - Azide Free

Western Blot: Aquaporin-2 [p Ser261] Antibody [NB100-61100]

Western Blot: Aquaporin-2 [p Ser261] Antibody [NB100-61100]

Western Blot: Aquaporin-2 [p Ser261] Antibody [NB100-61100] - Rat kidney lysate showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser261. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown).
Aquaporin-2 [p Ser261] Antibody - Azide Free

Western Blot: Aquaporin-2 [p Ser261] Antibody - Azide Free [NB100-61100] -

(A) Expression of AQP2 in 1 week HU and REST mice. Kidneys from HU and REST mice were lysed, and immunoblotting experiments were performed using specific antibodies against total AQP2. Densitometric analysis of total AQP2 bands normalized to the total protein content is reported in the histogram. Data are expressed as mean +/- S.E.M. (O.D.). Statistical analysis was done using Unpaired t-test (*p < 0.05). (B) Expression of AQP2 in 1 week HU and REST mice. Kidneys from HU and REST mice were lysed, and immunoblotting experiments were performed using specific antibodies against total AQP2. Densitometric analysis of total AQP2 bands normalized to the total protein content is reported in the histogram. Data are expressed as mean +/- S.E.M. (O.D.). Statistical analysis was done using Unpaired t-test (**p < 0.01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40303591), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Aquaporin-2 [p Ser261] Antibody - Azide Free

Western Blot: Aquaporin-2 [p Ser261] Antibody - Azide Free [NB100-61100] -

(A) Expression of AQP2-pS261 in 4 weeks HU compared to REST mice. Kidneys from HU and REST mice were lysed, and immunoblotting experiments were performed using specific antibodies against phosphorylated AQP2 in Ser261 and total AQP2. Densitometric analysis of AQP2-pS261 bands normalized to total AQP2 is reported in the histogram. Data are expressed as mean +/- S.E.M. (O.D.). Statistical analysis was done using Unpaired t-test (*p < 0.05). (B) Localization of AQP2-pS261 and total AQP2. Immunofluorescence staining of AQP2-pS261, in green (AlexaFluor 488), and total AQP2, in red (AlexaFluor 555), in renal sections of HU and REST mice (scale bar: 5 μm). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40303591), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Aquaporin-2 [p Ser261] Antibody - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 - 1:3000

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

3 uL

Western Blot

1:1000
Application Notes
Immunohistochemistry as reported in the literature (Hoffert et al., 2007) Dot Blot was reported in scientific literature.

Reviewed Applications

Read 1 review rated 5 using NB100-61100 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aquaporin-2

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein aquaporins. Aquaporin 0, and Aquaporin 1 have been the foundation of the growing family of aquaporins. The lens specific Aquaporin 0 represents up to 80% of total lens membrane protein. Defects in Aquaporin 0 are a cause of autosomal dominant cataract. The lens opacity mutation is an AA substitution that inhibits targeting of MIP to the cell membrane. Human Aquaporin 0 is a 263 amino acid transmembrane protein belonging to the MIP family. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N and C terminus are predicted to be cytoplasmic. Aquaporin 2 is located in the collecting tubule.

Alternate Names

ADH water channel, AQP-2, AQP-CD, aquaporin 2 (collecting duct), aquaporin-2, aquaporin-CD, Collecting duct water channel protein, MGC34501, Water channel protein for renal collecting duct, water-channel aquaporin 2, WCH-CD

Entrez Gene IDs

359 (Human); 11827 (Mouse); 25386 (Rat)

Gene Symbol

AQP2

UniProt

P34080 (Rat)

Additional Aquaporin-2 Products

Product Documents for Aquaporin-2 [p Ser261] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for Aquaporin-2 [p Ser261] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Aquaporin-2 [p Ser261] Antibody - Azide Free

Customer Reviews for Aquaporin-2 [p Ser261] Antibody - Azide Free (1)

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  • Name: Grazia Tamma
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 04/06/2016
    Aquaporin-2 [p Ser261] Antibody - Azide Free NB100-61100

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