Aquaporin-4 Antibody - BSA Free

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• Aquaporin-4 Antibody

  Name: Anonymous

  Application: Immunohistochemistry-Paraffin

  Sample Tested: Brain (cerebral cortex)

  Species: Human

  Verified Customer | Posted 12/24/2020

  AQP4 rabbit (1:2500) was incubated overnight at 4C on human cerebral cortex and visualised using AF Donkey anti-rabbit 488 (green).

  

• Aquaporin-4 Antibody

  Name: Kevin Thorburn

  Application: Immunohistochemistry-Frozen

  Sample Tested: Trigeminal ganglia

  Species: Mouse

  Verified Customer | Posted 11/22/2018

  Mouse trigeminal ganglion stained with aquaporin-4 antibody (1:5000 dilution).

  Mice were perfused with 0.9% saline and subsequently decapitated. The cerebrum was then dissected out of the cranium and the head was put in ~10mL of 4% PFA in 0.1M phosphate buffer overnight. The following morning the trigeminal ganglia were dissected and placed in 30% sucrose (sucrose dissolved in 0.1M phosphate buffer) for at least 48hr. The tissue was then blocked in OCT, frozen, cut at 10 microns (on a cryostat) and mounted on glass slides. Slides were washed three times with 1X PBS (diluted from a 10X stock (recipe available on thelabrat.com)) and blocked for 1hr in 1X PBS containing 0.2% triton X-100 and 10% goat serum. After the block, tissue was incubated overnight at room temperature in 1X PBS containing 0.2% triton X-100, 2% goat serum, 20mg/mL bovine serum albumin and the primary antibody diluted to a final concentration of 1:5000. The following morning the slides were washed twice with 1X PBS containing 0.2% tween-20 and once with 1X PBS. The slides were then incubated for 45 min (room temperature) in 1X PBS containing 0.2% triton X-100, 2% goat serum, 20mg/mL bovine serum albumin and 488-conjugated goat-anti rabbit secondary (ThermoFisher, 1:200). After the 45 min incubation period, slides were washed with washed twice with 1X PBS containing 0.2% tween 20 and once with 1X PBS. The slides were then coverslipped and mounted using vectashield mounting medium.

  

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