ARF1 Antibody

Novus Biologicals | Catalog # NBP2-29906

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all ARF1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide DWLSNQLRNQK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARF1 Antibody

Western Blot: ARF1 Antibody [NBP2-29906]

Western Blot: ARF1 Antibody [NBP2-29906]

Western Blot: ARF1 Antibody [NBP2-29906] - Analysis of 25ug of various whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel.
Immunocytochemistry/ Immunofluorescence: ARF1 Antibody [NBP2-29906]

Immunocytochemistry/ Immunofluorescence: ARF1 Antibody [NBP2-29906]

Immunocytochemistry/Immunofluorescence: ARF1 Antibody [NBP2-29906] - Analysis of Arf1 in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with an Arf1 polyclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.
Immunohistochemistry-Paraffin: ARF1 Antibody [NBP2-29906]

Immunohistochemistry-Paraffin: ARF1 Antibody [NBP2-29906]

Immunohistochemistry-Paraffin: ARF1 Antibody [NBP2-29906] - Analysis showing staining in the cytoplasm of human breast carcinoma (right) compared to a negative control without primary antibody (left).
Immunohistochemistry-Paraffin: ARF1 Antibody [NBP2-29906]

Immunohistochemistry-Paraffin: ARF1 Antibody [NBP2-29906]

Immunohistochemistry-Paraffin: ARF1 Antibody [NBP2-29906] - Analysis showing staining in the cytoplasm of mouse colon tissue (right) compared to a negative control without primary antibody (left).
Immunohistochemistry-Paraffin: ARF1 Antibody [NBP2-29906]

Immunohistochemistry-Paraffin: ARF1 Antibody [NBP2-29906]

Immunohistochemistry-Paraffin: ARF1 Antibody [NBP2-29906] - Analysis of showing staining in the cytoplasm of human colon carcinoma (right) compared to a negative control without primary antibody (left).
Immunoprecipitation: ARF1 Antibody [NBP2-29906]

Immunoprecipitation: ARF1 Antibody [NBP2-29906]

Immunoprecipitation: ARF1 Antibody [NBP2-29906] - Analysis of Arf1 was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug of HeLa whole cell lysate with 2ug of an Arf1 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples, including the HeLa cell lysate as a positive control (left lane), were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a nitrocellulose membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Arf1 polyclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP detection reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.

Applications for ARF1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:200

Immunohistochemistry

1:10 - 1:100

Immunohistochemistry-Paraffin

1:10 - 1:100

Immunoprecipitation

2 ug

Western Blot

1:1000 - 1:2000
Application Notes
Successful WB usage in human, mouse, and canine samples. Successful IF and IP usage in human samples.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA and 30% glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARF1

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.

Long Name

ADP-ribosylation factor 1

Alternate Names

ADP-ribosylation factor 1

Entrez Gene IDs

375 (Human); 11840 (Mouse)

Gene Symbol

ARF1

UniProt

P84077 (Human), P84075 (Mouse)

Additional ARF1 Products

Product Documents for ARF1 Antibody

Certificate of Analysis

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Product Specific Notices for ARF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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