ARFIP2 Antibody (2B5) - Azide and BSA Free

Novus Biologicals | Catalog # H00023647-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2B5

Format

Azide and BSA Free
View all ARFIP2 Primary Antibodies »

Product Specifications

Immunogen

ARFIP2 (AAH00392, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTDGILGKAATMEIPIHGNGEARQLPEDDGLEQDLQQVMVSGPNLNETSIVSGGYGGSGDGLIPTGSGRHPSHSTTPSGPGDEVARGIAGEKFDIVKKWGINTYKCTKQL

Reactivity Notes

Human. Other species not tested.

Localization

Cytoplasmic

Specificity

ARFIP2 - ADP-ribosylation factor interacting protein 2 (arfaptin 2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for ARFIP2 Antibody (2B5) - Azide and BSA Free

Western Blot: ARFIP2 Antibody (2B5) [H00023647-M01]

Western Blot: ARFIP2 Antibody (2B5) [H00023647-M01]

Western Blot: ARFIP2 Antibody (2B5) [H00023647-M01] - ARFIP2 monoclonal antibody (M01), clone 2B5 Analysis of ARFIP2 expression in IMR-32.
Sandwich ELISA: ARFIP2 Antibody (2B5) [H00023647-M01] - Detection limit for recombinant GST tagged ARFIP2 is 0.03 ng/ml as a capture antibody.

Applications for ARFIP2 Antibody (2B5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARFIP2

ARFIP2 is a ubiquitously expressed protein implicated in mediating cross talk between RAC and ARF small GTPases. It has been shown that ARFIP2 binds specifically to GTP-bound ARF1 and ARF6, but binds to Rac-GTP and Rac-GDP with similar affinities. The X-ray structure of arfaptin reveals an elongated, crescent-shaped dimer of 3-helix coiled-coils. Structures of arfaptin with Rac bound to either GDP or the slowly hydrolysable analog GMPPNP show that the switch regions adopt similar conformations in both complexes.

Alternate Names

ADP-ribosylation factor interacting protein 2, ADP-ribosylation factor-interacting protein 2, arfaptin 2, arfaptin-2, Partner of RAC1, POR1partner of RAC1 (arfaptin 2), Protein POR1

Entrez Gene IDs

23647 (Human)

Gene Symbol

ARFIP2

OMIM

601638 (Human)

UniProt

AAH00392 (Human)

Additional ARFIP2 Products

Product Documents for ARFIP2 Antibody (2B5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ARFIP2 Antibody (2B5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ARFIP2 Antibody (2B5) - Azide and BSA Free

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Protocols

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FAQs

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