Argininosuccinate Synthase Antibody (2B10) - BSA Free

Novus Biologicals | Catalog # NBP2-37520

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2B10

Format

BSA Free
View all Argininosuccinate Synthase Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Argininosuccinate Synthase expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Argininosuccinate Synthase Antibody (2B10) - BSA Free

Western Blot: Argininosuccinate Synthase Antibody (2B10)BSA Free [NBP2-37520]

Western Blot: Argininosuccinate Synthase Antibody (2B10)BSA Free [NBP2-37520]

Western Blot: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Analysis using ASS1 mouse mAb against A431 (1), RAJI (2), L1210 (3), MOLT4 (4), Jurkat (5), A549 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: Argininosuccinate Synthase Antibody (2B10) - BSA Free [NBP2-37520]

Immunocytochemistry/ Immunofluorescence: Argininosuccinate Synthase Antibody (2B10) - BSA Free [NBP2-37520]

Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Analysis of Hela cells using ASS1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry: Argininosuccinate Synthase Antibody (2B10) - BSA Free [NBP2-37520]

Flow Cytometry: Argininosuccinate Synthase Antibody (2B10) - BSA Free [NBP2-37520]

Flow Cytometry: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Analysis of Jurkat cells using ASS1 mouse mAb (green) and negative control (red).
Western Blot: Argininosuccinate Synthase Antibody (2B10)BSA Free [NBP2-37520]

Western Blot: Argininosuccinate Synthase Antibody (2B10)BSA Free [NBP2-37520]

Western Blot: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Analysis using ASS1 mAb against human ASS1 (AA: 40-236) recombinant protein. (Expected MW is 47 kDa)
ELISA: Argininosuccinate Synthase Antibody (2B10) - BSA Free [NBP2-37520]

ELISA: Argininosuccinate Synthase Antibody (2B10) - BSA Free [NBP2-37520]

ELISA: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Argininosuccinate Synthase Antibody (2B10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Argininosuccinate Synthase

Argininosuccinate Synthase is encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of Argininosuccinate Synthase cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Alternate Names

argininosuccinate synthase, argininosuccinate synthase 1, argininosuccinate synthetase, argininosuccinate synthetase 1, ASSCTLN1, citrulline-aspartate ligase, Citrulline--aspartate ligase, EC 6.3.4.5

Gene Symbol

ASS1

Additional Argininosuccinate Synthase Products

Product Documents for Argininosuccinate Synthase Antibody (2B10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Argininosuccinate Synthase Antibody (2B10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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