Argonaute 3 Antibody (PCRP-AGO3-1C5)
Novus Biologicals | Catalog # NBP3-13873
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PCRP-AGO3-1C5
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Product Specifications
Immunogen
Recombinant full-length human Argonaute 3 protein (Uniprot: Q9H9G7)
Localization
Cytoplasm, P-bodies, Nucleoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
97.36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14202)
Antibody with azide store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Argonaute 3 Antibody (PCRP-AGO3-1C5)
![Immunocytochemistry/ Immunofluorescence: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873]](https://resources.rndsystems.com/images/products/Argonaute-3-Antibody-PCRP-AGO3-1C5-Immunocytochemistry-Immunofluorescence-NBP3-13873-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873]")
Immunocytochemistry/ Immunofluorescence: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873]
Immunocytochemistry/Immunofluorescence: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873] - Immunofluorescence Analysis of PFA-fixed HeLa cells. Cytoplasmic P-bodies and nucleoplasm stained using Argonaute 3 Antibody (PCRP-AGO3-1C5) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).![Flow Cytometry: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873]](https://resources.rndsystems.com/images/products/Argonaute-3-Antibody-PCRP-AGO3-1C5-Flow-Cytometry-NBP3-13873-img0001.jpg "Flow Cytometry: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873]")
Flow Cytometry: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873]
Flow Cytometry: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873] - Flow cytometric analysis of PFA-fixed HeLa cells. Argonaute 3 antibody (PCRP-AGO3-1C5) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).![Protein Array: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873]](https://resources.rndsystems.com/images/products/Argonaute-3-Antibody-PCRP-AGO3-1C5-Protein-Array-NBP3-13873-img0004.jpg "Protein Array: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873]")
Protein Array: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873]
Protein Array: Argonaute 3 Antibody (PCRP-AGO3-1C5) [NBP3-13873] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Argonaute 3 Antibody (PCRP-AGO3-1C5).Applications for Argonaute 3 Antibody (PCRP-AGO3-1C5)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunoprecipitation
1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Argonaute 3
Alternate Names
AGO3argonaute 3, Argonaute3, eIF2C 3, eIF-2C 3, Eukaryotic translation initiation factor 2C 3, eukaryotic translation initiation factor 2C, 3, FLJ12765, hAgo3, MGC86946, protein argonaute-3
Gene Symbol
AGO3
UniProt
Additional Argonaute 3 Products
Product Documents for Argonaute 3 Antibody (PCRP-AGO3-1C5)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Argonaute 3 Antibody (PCRP-AGO3-1C5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Appropriate Fixation of IHC/ICC Samples
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- ELISA Sample Preparation & Collection Guide
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- ICC Immunocytochemistry Protocol Videos
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- Immunocytochemistry (ICC) Protocol
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- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
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- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
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- Preparing Samples for IHC/ICC Experiments
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- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
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- Protocol for the Characterization of Human Th22 Cells
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- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
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- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
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- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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