ARHGEF5 Antibody (3A12-B5) - Azide and BSA Free

Novus Biologicals | Catalog # H00007984-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3A12-B5

Format

Azide and BSA Free
View all ARHGEF5 Primary Antibodies »

Product Specifications

Immunogen

ARHGEF5 (AAH14555, 1 a.a. ~ 519 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSETPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELIVSEASYLRSLNIAVDHFQLSTSLRATLSNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSSEEAEATKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTALEFSASPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLRQNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDGERGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQA

Specificity

ARHGEF5 - Rho guanine nucleotide exchange factor (GEF) 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ARHGEF5 Antibody (3A12-B5) - Azide and BSA Free

Western Blot: ARHGEF5 Antibody (3A12-B5) [H00007984-M01]

Western Blot: ARHGEF5 Antibody (3A12-B5) [H00007984-M01]

Western Blot: ARHGEF5 Antibody (3A12-B5) [H00007984-M01] - Analysis of ARHGEF5 expression in transfected 293T cell line by ARHGEF5 monoclonal antibody (M01), clone 3A12-B5.Lane 1: ARHGEF5 transfected lysate(60.1 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: ARHGEF5 Antibody (3A12-B5) [H00007984-M01] - Detection limit for recombinant GST tagged ARHGEF5 is 1 ng/ml as a capture antibody.

Applications for ARHGEF5 Antibody (3A12-B5) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARHGEF5

Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization.

Alternate Names

Ephexin-3, GEF5, Guanine nucleotide regulatory protein TIM, Oncogene TIM, P60, p60 TIM, Rho guanine nucleotide exchange factor (GEF) 5, rho guanine nucleotide exchange factor 5, TIM1, TIMDKFZp686N1969, Transforming immortalized mammary oncogene

Entrez Gene IDs

7984 (Human)

Gene Symbol

ARHGEF5

OMIM

600888 (Human)

UniProt

AAH14555 (Human)

Additional ARHGEF5 Products

Product Documents for ARHGEF5 Antibody (3A12-B5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ARHGEF5 Antibody (3A12-B5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ARHGEF5 Antibody (3A12-B5) - Azide and BSA Free

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Protocols

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FAQs

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