ARID1B Antibody

Novus Biologicals | Catalog # NB100-57484

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all ARID1B Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1300 and 1350 of human AT rich interactive domain 1B using the numbering given in entry NP_787059.2 (GeneID 57492).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARID1B Antibody

Western Blot: ARID1B Antibody [NB100-57484]

Western Blot: ARID1B Antibody [NB100-57484]

Western Blot: ARID1B Antibody [NB100-57484] - Detection of human ARID1B by western blot. Samples: Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID1B antibody NB100-57484 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: ARID1B Antibody [NB100-57484]

Immunohistochemistry-Paraffin: ARID1B Antibody [NB100-57484]

Immunohistochemistry-Paraffin: ARID1B Antibody [NB100-57484] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ARID1B (NB100-57484). Detection: DAB
Western Blot: ARID1B Antibody [NB100-57484]

Western Blot: ARID1B Antibody [NB100-57484]

Western Blot: ARID1B Antibody [NB100-57484] - Detection of Human ARID1B on HeLa whole cell lysates using NB100-57484. ARID1B was also immunoprecipitated by rabbit anti-ARID1B antibody NB100-47485.
Immunoprecipitation: ARID1B Antibody [NB100-57484]

Immunoprecipitation: ARID1B Antibody [NB100-57484]

Immunoprecipitation: ARID1B Antibody [NB100-57484] - Detection of human ARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID1B antibody NB100-57484 (lot NB100-57484-3) used for IP at 3 ug per reaction. ARID1B was also immunoprecipitated by a previous lot of this antibody (lot NB100-57484-2) and rabbit anti-ARID1B antibody NB100-57485. For blotting immunoprecipitated ARID1B, NB100-57484 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for ARID1B Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ARID1B

ARID1B (AT-rich interactive domain-containing protein 1B) is a component of SWI/SNF chromatin remodeling complexes involved in transcriptional activation and repression of select genes by chromatin remodeling. It binds DNA non-specifically and alters DNA-nucleosome topology.

Alternate Names

6A3-5, ARID domain-containing protein 1B, AT rich interactive domain 1B (SWI1-like), BAF250b, BRG1-associated factor 250b, BRG1-binding protein ELD/OSA1, BRG1-binding protein hELD/OSA1, BRIGHT, DAN15P250R, ELD (eyelid)/OSA protein, ELD/OSA1, hOsa2, JPUT1, KDM5B, KIAA1235AT-rich interactive domain-containing protein 1B, Osa homolog 2, OSA2, p250R, PLU1, RBBP2H1A

Entrez Gene IDs

57492 (Human)

Gene Symbol

ARID1B

UniProt

Q8NFD5 (Human)

Additional ARID1B Products

Product Documents for ARID1B Antibody

Certificate of Analysis

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Product Specific Notices for ARID1B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ARID1B Antibody

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Protocols

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