ARID5A Antibody (18112) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43745

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 18112

Format

Azide and BSA Free
View all ARID5A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARID5A. The exact sequence is proprietary.

Reactivity Notes

Bovine (88%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARID5A Antibody (18112) - Azide and BSA Free

Western Blot: ARID5A Antibody (18112) [NBP2-43745]

Western Blot: ARID5A Antibody (18112) [NBP2-43745]

Western Blot: ARID5A Antibody (18112) [NBP2-43745] - Analysis of Non-transfected (-) and ARID5A-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody diluted by 1:5000.
Western Blot: ARID5A Antibody (18112) [NBP2-43745]

Western Blot: ARID5A Antibody (18112) [NBP2-43745]

Western Blot: ARID5A Antibody (18112) [NBP2-43745] - Untreated (-) and treated (+) Raw264.7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody 18112]. The signal was developed with Trident ECL plus-Enhanced. (LPS: Lipopolysaccharides)
ARID5A Antibody (18112) - Azide and BSA Free

Immunohistochemistry-Frozen: ARID5A Antibody (18112) [NBP2-43745] -

ARID5A antibody [GT18112] detects ARID5A protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse mouse cerebellum.
Green: ARID5A stained by ARID5A antibody [GT18112] (NBP2-43745) diluted at 1:250.
Red: Calbindin, stained by Calbindin antibody diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
ARID5A Antibody (18112) - Azide and BSA Free

Western Blot: ARID5A Antibody (18112) [NBP2-43745] -

THP-1 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody [GT18112] (NBP2-43745) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
ARID5A Antibody (18112) - Azide and BSA Free

Flow Cytometry: ARID5A Antibody (18112) [NBP2-43745] -

ARID5A antibody [GT18112] (NBP2-43745) detects ARID5A protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: ARID5A antibody [GT18112] (NBP2-43745) dilution: 1:20.
Acquisition of 20,000 events were collected for FACS analysis.

Applications for ARID5A Antibody (18112) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:20-1:200

Immunohistochemistry-Frozen

1:100-1:1000

Western Blot

1:500-1:10000

Reviewed Applications

Read 1 review rated 3 using NBP2-43745 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARID5A

Members of the ARID protein family, including ARID5A, have diverse functions but all appear to play important roles indevelopment, tissue-specific gene expression, and regulation of cell growth (Patsialou et al., 2005 (PubMed15640446)).(supplied by OMIM)

Alternate Names

ARID domain-containing protein 5A, AT rich interactive domain 5A (MRF1-like), AT-rich interactive domain-containing protein 5A, RFVG5814

Gene Symbol

ARID5A

Additional ARID5A Products

Product Documents for ARID5A Antibody (18112) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ARID5A Antibody (18112) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ARID5A Antibody (18112) - Azide and BSA Free (1)

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  • ARID5A Antibody (18112)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: AC16
    Species: Human
    Verified Customer | Posted 05/07/2020
    ARID5a
    1:5000 dilution
    ARID5A Antibody (18112) - Azide and BSA Free NBP2-43745

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