ASC/TMS1 Antibody (OTI3E9)

Novus Biologicals | Catalog # NBP2-03818

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3E9
View all ASC Primary Antibodies »

Product Specifications

Immunogen

This ASC/TMS1 Antibody (OTI3E9) was developed against full length human recombinant protein of human ASC/TMS1 (NP_037390) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ASC/TMS1 Antibody (OTI3E9)

Western Blot: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818]

Western Blot: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818]

Western Blot: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-ASC/TMS1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818]

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818]

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Staining of paraffin-embedded Human liver tissue using anti-ASC/TMS1 mouse monoclonal antibody.
Flow Cytometry: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818]

Flow Cytometry: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818]

Flow Cytometry: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ASC/TMS1 antibody, and then analyzed by flow cytometry.
Western Blot: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818]

Western Blot: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818]

Western Blot: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASC/TMS1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASC/TMS1.
Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818]

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818]

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ASC/TMS1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818]

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818]

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ASC/TMS1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818]

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818]

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ASC/TMS1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818]

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818]

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Staining of paraffin-embedded Human colon tissue using anti-ASC/TMS1 mouse monoclonal antibody.

Applications for ASC/TMS1 Antibody (OTI3E9)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000
Application Notes
For IHC-P, antigen retrieval is recommended with 10mM citric buffer, pH6.0, 100C for 10min.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ASC

ASC (apoptosis-associated speck-like protein containing a CARD), also known as TMS1 (target of methylation-induced silencing), was first identified in 1999 as a protein that forms aggregates, or specks, during retinoic acid-induced apoptosis in a human leukemia cell line (1). Furthermore, it was discovered to have a role as a tumor suppressor as methylation silences ASC/TMS1 expression in many tumors (2-5). ASC/TMS1 is synthesized as a 195-amino acid (aa) protein with a theoretical weight of 22 kDa. Structurally the protein contains a N-terminal PYD (pyrin domain) and C-terminal CARD (caspase-recruitment domain) (1-4). Historically, CARD and PYD-containing proteins are known to have crucial functions in regulating apoptosis and immune response pathways (2-5). Furthermore, mutations in many CARD and PYD-containing proteins have been linked to various cancers and inflammatory diseases (2-5). Given its immune response role, it is not surprising that ASC/TMS1 is typically highly expressed in immune cells, specifically in neutrophils and cells of the macrophage/monocyte lineage (5). Additionally, it is expressed in many normal epithelial cell types (4,5).

In regard to immune and inflammatory response, ASC/TMS1 is involved in inflammasome function (3-4). The inflammasome is a multiprotein complex that responds to cellular stress or pathogens and activates inflammatory responses. Specifically, ASC/TMS1 helps assemble the NLRP3 inflammasome complex which then activates caspase-1, followed by stimulation of proinflammatory cytokines including IL-1b and IL-18 (3-4). In terms of the role in regulating apoptosis, multiple studies have revealed that the ASC/TMS1 gene is hypermethylated in many cancers including breast, lung, glioblastomas, and melanomas (2-5). The increased methylation results in decreased gene expression, or silencing, allowing those cancer cells to escape apoptosis (2-5).

References

1. Masumoto, J., Taniguchi, S., Ayukawa, K., Sarvotham, H., Kishino, T., Niikawa, N., Hidaka, E., Katsuyama, T., Higuchi, T., & Sagara, J. (1999). ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells. The Journal of biological chemistry, 274(48), 33835-33838. https://doi.org/10.1074/jbc.274.48.33835

2. McConnell, B. B., & Vertino, P. M. (2004). TMS1/ASC: the cancer connection. Apoptosis: an international journal on programmed cell death. https://doi.org/10.1023/B:APPT.0000012117.32430.0c

3. Salminen, A., Kauppinen, A., Hiltunen, M., & Kaarniranta, K. (2014). Epigenetic regulation of ASC/TMS1 expression: potential role in apoptosis and inflammasome function. Cellular and molecular life sciences : CMLS. https://doi.org/10.1007/s00018-013-1524-9

4. Protti, M. P., & De Monte, L. (2020). Dual Role of Inflammasome Adaptor ASC in Cancer. Frontiers in cell and developmental biology. https://doi.org/10.3389/fcell.2020.00040

5. Parsons, M. J., & Vertino, P. M. (2006). Dual role of TMS1/ASC in death receptor signaling. Oncogene. https://doi.org/10.1038/sj.onc.1209684

Long Name

Apoptosis-Associated Speck-Like Protein Containing A CARD

Alternate Names

CARD5, PYCARD, TMS1

Entrez Gene IDs

29108 (Human)

Gene Symbol

PYCARD

Additional ASC Products

Product Documents for ASC/TMS1 Antibody (OTI3E9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ASC/TMS1 Antibody (OTI3E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ASC/TMS1 Antibody (OTI3E9)

Customer Reviews for ASC/TMS1 Antibody (OTI3E9)

There are currently no reviews for this product. Be the first to review ASC/TMS1 Antibody (OTI3E9) and earn rewards!

Have you used ASC/TMS1 Antibody (OTI3E9)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ASC/TMS1 Antibody (OTI3E9)

Showing  1 - 1 of 1 FAQ Showing All

  • Q: Please advise the antigen retrieval conditions/procedure that you recommend.

    A: For this item our lab recommends performing heat induced antigen retrieval with 10mM citric buffer, pH6.0, 100C for 10min.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies