ASCC2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88815

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ASCC2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YSVVVEEVPLQPGESLPYHSVYYEDEYDDTYDGNQVGANDADSDDELISRRPFTIPQVLRTKVPREGQEEDDDDEEDDADEEAPKPDHFVQDPAVLREKAEARRMAFLAKKGYRHDSSTAVAGSPRGHGQSRETTQERRKKEANKATRANH

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASCC2 Antibody - BSA Free

Western Blot: ASCC2 Antibody [NBP1-88815]

Western Blot: ASCC2 Antibody [NBP1-88815]

Western Blot: ASCC2 Antibody [NBP1-88815] - Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line EFO-21. Lane 4: Human cell line A-431. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: ASCC2 Antibody [NBP1-88815]

Immunocytochemistry/ Immunofluorescence: ASCC2 Antibody [NBP1-88815]

Immunocytochemistry/Immunofluorescence: ASCC2 Antibody [NBP1-88815] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, cytosol & focal adhesion sites.
Immunohistochemistry-Paraffin: ASCC2 Antibody [NBP1-88815]

Immunohistochemistry-Paraffin: ASCC2 Antibody [NBP1-88815]

Immunohistochemistry-Paraffin: ASCC2 Antibody [NBP1-88815] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Applications for ASCC2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASCC2

Enhances NF-kappa-B, SRF and AP1 transactivation

Alternate Names

activating signal cointegrator 1 complex subunit 2, ASC 1 complex subunit P100, ASC-1 complex subunit p100, ASC1p100, DKFZp586O0223, FLJ21588, Trip4 complex subunit p100

Gene Symbol

ASCC2

Additional ASCC2 Products

Product Documents for ASCC2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ASCC2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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