ASCL1/Mash1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-13159

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ASCL1/Mash1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse ASCL1/Mash1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for ASCL1/Mash1 Antibody - BSA Free

ASCL1/Mash1 Antibody

Western Blot: ASCL1/Mash1 Antibody [NBP3-13159] -

Western Blot: ASCL1/Mash1 Antibody [NBP3-13159] - SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1/Mash1 antibody (NBP3-13159) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ASCL1/Mash1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ASCL1/Mash1 Antibody [NBP3-13159] -

ASCL1 antibody detects ASCL1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASCL1 stained by ASCL1 antibody (NBP3-13159) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for ASCL1/Mash1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASCL1/Mash1

MASH1 encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.

Long Name

Achaete Scute Complex-like 1

Alternate Names

ASCL1, ASH1, HASH1, Mash1

Gene Symbol

ASCL1

Additional ASCL1/Mash1 Products

Product Documents for ASCL1/Mash1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ASCL1/Mash1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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