ASCL3 Antibody
Novus Biologicals | Catalog # NBP1-68871
![Western Blot: ASCL3 Antibody [NBP1-68871]](https://resources.rndsystems.com/images/products/ASCL3-Antibody-Western-Blot-NBP1-68871-img0002.jpg "Western Blot: ASCL3 Antibody [NBP1-68871]")
Loading...
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Loading...
Product Specifications
Immunogen
Peptide with sequence C-HLPEDYLEKRLSK corresponding to internal region according to Uniprot Mouse NP_064435.1.
Epitope
C-HLPEDYLEKRLSK
Reactivity Notes
Expected from sequence similarity: Bovine
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for ASCL3 Antibody
Western Blot: ASCL3 Antibody [NBP1-68871]
Western Blot: ASCL3 Antibody [NBP1-68871] - (0.1ug/ml) Analysis of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: ASCL3 Antibody [NBP1-68871]](https://resources.rndsystems.com/images/products/ASCL3-Antibody-Immunohistochemistry-Paraffin-NBP1-68871-img0003.jpg "Immunohistochemistry-Paraffin: ASCL3 Antibody [NBP1-68871]")
Immunohistochemistry-Paraffin: ASCL3 Antibody [NBP1-68871]
Immunohistochemistry-Paraffin: ASCL3 Antibody [NBP1-68871] - 3.75ug/ml staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for ASCL3 Antibody
Application
Recommended Usage
Immunohistochemistry
0.1-0.3ug/ml
Peptide ELISA
detection limit 1:8000
Western Blot
0.1 ug/ml
Application Notes
Western blot: Approx 23kDa band observed in Mouse Liver lysates (calculated MW of 20.2kDa according to NP_064435.1). Primary incubation was 1 hour
IHC: Paraffin embedded Human Brain (Cortex).
IHC: Paraffin embedded Human Brain (Cortex).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ASCL3
Alternate Names
achaete-scute complex homolog 3 (Drosophila), ASH-3, bHLH transcription factor Sgn-1 (Salivary Glands 1), bHLH transcriptional regulator Sgn-1, BHLHA42, bHLHa42achaete-scute homolog 3, Class A basic helix-loop-helix protein 42, hASH3, HASH3achaete-scute complex (Drosophila) homolog-like 3, SGN1
Gene Symbol
ASCL3
UniProt
Additional ASCL3 Products
Product Documents for ASCL3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ASCL3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for ASCL3 Antibody
Powered by Bioz
Customer Reviews for ASCL3 Antibody
There are currently no reviews for this product. Be the first to review ASCL3 Antibody and earn rewards!
Have you used ASCL3 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...