ASGPR1 Antibody (8D7)

Novus Biologicals | Catalog # NBP1-60150

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8D7
View all ASGR1/ASGPR1 Primary Antibodies »

Product Specifications

Immunogen

Crude rat liver membrane extracts

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33554615).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ASGPR1 Antibody (8D7)

Flow Cytometry: ASGPR1 Antibody (8D7) [NBP1-60150]

Flow Cytometry: ASGPR1 Antibody (8D7) [NBP1-60150]

Flow Cytometry: ASGPR1 Antibody (8D7) [NBP1-60150] - Detection of ASGPR1 in HepG2 cells. Red, black and blue line represent the isotype control, cells only, and an ASGPR1 antibody conjugated to FITC with a concentration of 10 ug/ml, respectively.

Applications for ASGPR1 Antibody (8D7)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This antibody can be used in Flow Cytometry, ELISA, Immunofluorescence, Immunohistochemistry-Frozen and Western Blot. Western Blot: it is recommended to run under non-reducing conditions. The band size is approximately 42 kDa. Immunohistochemistry-Frozen: acetone fixation is recommended. ELISA: it is recommended to coat microtiter plates with antibody at 20ug/ml. Recommended controls: Positive: Rat hepatocytes; Negative: Rat kidney, pancreas, small intestine, and colon. Dilutions to be used depend on the detection system that is applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Reviewed Applications

Read 1 review rated 4 using NBP1-60150 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS and 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ASGR1/ASGPR1

The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid residues (asialoglycoproteins). Characterization of the ASGP receptor revealed its functional role in the binding, internalization and transport of a wide range of glycoproteins, which have exposed galactose or N-acetylgalactosamine residues, via the process of receptor-mediated endocytosis (RME). The ASGP receptor can bind a variety of important plasma proteins including transport proteins (i.e. transferrin), enzymes such as alkaline phosphatase, immunoglobulins including IgA, apoptotic hepatocytes, fibronectin and platelets. Additionally, the expression of the ASGP receptor has been clinically correlated to the level of hepatic function that is lost during liver diseases related to cancer, viral hepatitis, and cirrhosis. The ASGP receptor consists of major and minor subunits, which in the rat were identified as rat hepatic lectin (RHL) 1 and RHL 2/3, with molecular weights of respectively 42, 49 and 54 kDa. The selective binding (calcium and pH depended) and uptake of terminal galactosyl bearing proteins requires the formation of hetero-oligomers between these major and minor forms The total ASGP receptor population consisted of two functionally distinct receptor populations, designated State 1 and State 2, which were involved in the endocytosis and intracellular processing of ligands by different pathways. The monoclonal antibody 8D7 recognizes a subunit-specific epitope on RHL-1 of rat ASGPR. The monoclonal antibody 8D7 is cross reactive with human ASGPR.

Long Name

Asialoglycoprotein Receptor 1

Alternate Names

ASGPR1, CLEC4H1, MHL1, RHL1

Gene Symbol

ASGR1

Additional ASGR1/ASGPR1 Products

Product Documents for ASGPR1 Antibody (8D7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ASGPR1 Antibody (8D7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

C-type Lectin Family

Citations for ASGPR1 Antibody (8D7)

Customer Reviews for ASGPR1 Antibody (8D7) (1)

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Customer Images

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  • RARRES3 Antibody
    Name: Armen Petrosyan
    Application: Immunofluorescence
    Sample Tested: Mouse Liver cells
    Species: Mouse
    Verified Customer | Posted 10/06/2021
    Image was taken by EVOS 5000 using 60x oil objective
    ASGPR1 Antibody (8D7) NBP1-60150

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Protocols

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FAQs for ASGPR1 Antibody (8D7)

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  • Q: I am looking for ASGPR1 or any surface marker for hepatocytes reactive to mouse and monoclonal for flow cytometry application. I found NBP1-60150-0.025mg in your webpage. I was wondering if it would be a good fit for my application.

    A: The antibody you are looking at (NBP1-60150-0.025mg) is validated and guaranteed for detection of mouse ASGPR1 in Flow. The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid residues (asialoglycoproteins). The ASGP receptor consists of major and minor subunits, which in the rat were identified as rat hepatic lectin (RHL) 1 and RHL 2/3, with molecular weights of respectively 42, 49 and 54 kDa. This monoclonal antibody (clone 8D7) recognizes a subunit-specific epitope on RHL-1 of rat ASGPR. The monoclonal antibody 8D7 is cross reactive with ASGPR from human, mouse and rat species.

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