ASK1 Antibody (2E4)

Novus Biologicals | Catalog # NBP2-37592

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2E4
View all ASK1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human ASK1 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse ASK1 Antibody (2E4) (NBP2-37592) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-ASK1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ASK1 Antibody (2E4)

Western Blot: ASK1 Antibody (2E4)BSA Free [NBP2-37592]

Western Blot: ASK1 Antibody (2E4)BSA Free [NBP2-37592]

Western Blot: ASK1 Antibody (2E4) [NBP2-37592] - Western blot analysis using MAP3K5 mAb against human MAP3K5 (AA: 922-1108) recombinant protein. (Expected MW is 46.5 kDa)
Immunohistochemistry: ASK1 Antibody (2E4) - BSA Free [NBP2-37592]

Immunohistochemistry: ASK1 Antibody (2E4) - BSA Free [NBP2-37592]

Immunohistochemistry: ASK1 Antibody (2E4) [NBP2-37592] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and prostate tissues (right) using MAP3K5 mouse mAb with DAB staining.
Flow Cytometry: ASK1 Antibody (2E4) - BSA Free [NBP2-37592]

Flow Cytometry: ASK1 Antibody (2E4) - BSA Free [NBP2-37592]

Flow Cytometry: ASK1 Antibody (2E4) [NBP2-37592] - Flow cytometric analysis of Hela cells using MAP3K5 mouse mAb (green) and negative control (purple).
Immunohistochemistry: ASK1 Antibody (2E4) - BSA Free [NBP2-37592]

Immunohistochemistry: ASK1 Antibody (2E4) - BSA Free [NBP2-37592]

Immunohistochemistry: ASK1 Antibody (2E4) [NBP2-37592] - Immunofluorescence analysis of Hela cells using MAP3K5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
ELISA: ASK1 Antibody (2E4) - BSA Free [NBP2-37592]

ELISA: ASK1 Antibody (2E4) - BSA Free [NBP2-37592]

ELISA: ASK1 Antibody (2E4) [NBP2-37592] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for ASK1 Antibody (2E4)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASK1

Apoptosis signal-regulating kinase-1 (ASK1, MEKK5, MAPKKK5) is a MAP3k type protein kinase belonging to mitogen-activated protein kinase kinase kinase (MAPKKK) family. As a regulator of JNK and p38 MAPK signaling pathways, ASK1/MEKK5 phosphorylates MMK4 to activate c-Jun N-terminal kinase (JNK) and phosphorylates MEK3 and MKK6 to activate p38. ASK1/MEKK5 is activated by oxidative stress, TNF via TRAF2, Fas via Daxx, calcium overload, and endoplasmic reticulum stress (1). ASK1/MEKK5 is inactivated by Trx, Grx, 14-3-3, and protein serine/threonine phosphatase 5 (2). While elevated level of ASK1/MEKK5 can induce apoptosis, a catalytically inactive ASK1/MEKK5 acts as an inhibitor in TNF-alpha induced apoptosis. Over-expression of ASK1/MEKK5 has also been implicated in cell differentiation, such as in keratinocyte differentiation through p38 pathway (3). ASK1/MEKK5 has been implicated as a therapeutic target for neurodegenerative disorder sand cardiac dysfunction (4).

Long Name

Apoptosis Signal-regulated Kinase 1

Alternate Names

MAP3K5, MEKK5

Gene Symbol

MAP3K5

Additional ASK1 Products

Product Documents for ASK1 Antibody (2E4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ASK1 Antibody (2E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ASK1 Antibody (2E4)

Customer Reviews for ASK1 Antibody (2E4)

There are currently no reviews for this product. Be the first to review ASK1 Antibody (2E4) and earn rewards!

Have you used ASK1 Antibody (2E4)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies

Associated Pathways

MAPK Signaling: Oxidative Stress Pathway MAPK Signaling: Oxidative Stress Pathway Thumbnail