ASPA Antibody - BSA Free

Western Blot: ASPA Antibody [NBP1-31754] -

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ASPA antibody [N1C3-2] (NBP1-31754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: ASPA Antibody [NBP1-31754] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ASPA antibody [N1C3-2] (NBP1-31754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: ASPA Antibody - BSA Free [NBP1-31754] -

NAA enhances lipid turnover and phagocytic activity in primary microglial cells treated for 1 week. (A) Representative Western blot of ASPA levels. beta -Actin was used as loading control. Bar graph (right) refers to the densitometry analysis (n = 3). (B) RT-qPCR analysis of HDAC1 mRNA. ACTB was used as reference gene. Data are shown as fold change vs. CTRL (n = 3; *p < 0.05 vs. CTRL). (C) Representative images of immunofluorescence analysis of lipid droplet content after Oil red O staining in microglial cells pre-treated with 200 uM NAA for 1 week and 40 uM ATGLi for 24 h. Bar graph (right) refers to the immunofluorescence quantification (n = 3; **p < 0.01; ***p < 0.001 as indicated). (D) RT-qPCR analysis of genes involved in beta -oxidation. ACTB was used as reference gene. Data are shown as fold change vs. CTRL, which was represented by a dashed line in the bar graph (n = 3; *p < 0.05 vs. CTRL). (E) Representative images and quantification (right) of phagocytic activity tested by using fluorescent latex beads (n = 3; *p < 0.05 vs. CTRL) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39587614), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ASPA Antibody - BSA Free [NBP1-31754] -

NAA enhances lipid metabolism and phagocytic activity in BV2 cells treated for 1 week. (A) Representative images of immunofluorescence analysis of lipid droplet content after Oil red O staining in BV2 cells treated for 24 h with 40 uM ATGLi. Bar graph (right) refers to the immunofluorescence quantification (n = 3; *p < 0.05; **p < 0.01; ***p < 0.001 as indicated). (B) RT-qPCR analysis of genes involved in beta -oxidation. ACTB was used as reference gene. Data are shown as fold change vs. CTRL, which was represented by a dashed line in the bar graph (n = 3; *p < 0.05 vs. CTRL). (C) Determination of NAA levels by HPLC analysis (n = 4; **p < 0.01 vs. CTRL). (D) Representative Western blot of ASPA levels. beta -Actin was used as loading control. Bar graph (below) refers to the densitometry analysis (n = 3). Determination of Acetyl-CoA (E) and Malonyl-CoA (F) levels by HPLC analysis (n = 4; **p < 0.01; ***p < 0.001 vs. CTRL). (G) Representative Western blot of HK2 and PKM2 levels. beta -Actin was used as loading control. Bar graph (below) refers to the densitometry analysis (n = 3; *p < 0.05 vs. CTRL). (H) Evaluation of extracellular lactate content normalized on total proteins (n = 3; * p < 0.05 vs. CTRL). (I) Representative images and quantification (right) of phagocytic activity using green fluorescent latex beads (n = 3; *p < 0.05 vs. CTRL) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39587614), licensed under a CC-BY license. Not internally tested by Novus Biologicals.