ATF2 [p Thr71] Antibody (103C411.2) - Azide and BSA Free

Name: ATF2 [p Thr71] Antibody (103C411.2) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-33236
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-33236

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 103C411.2

Format

Azide and BSA Free

View all ATF2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated by immunizing mice with a synthetic peptide containing phosphorylated threonine at position 71 of human ATF-2.

Modification

p Thr71

Specificity

This monoclonal antibody is specific for phospho (Thr71) ATF2. It cannot be used to test for non-phosphorylated ATF2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for ATF2 [p Thr71] Antibody (103C411.2) - Azide and BSA Free

Western Blot: ATF2 [p Thr71] Antibody (103C411.2) - Azide Free [NBP2-33236] - Detection of ATF2 phosphorylation in HeLa cell lysate with phospho ATF2 antibody. Lane A. Untreated cell lysate; Lane B. HeLa cells treated with TNF for 1 hr. Image from the standard format of this antibody.

Applications for ATF2 [p Thr71] Antibody (103C411.2) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

1ul/1 million cells

Western Blot

2 ug/ml

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATF2

ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Long Name

Activating Transcription Factor 2

Alternate Names

CRE-BP1, CREB2, HB16, mXBP, TREB7

Gene Symbol

ATF2

Additional ATF2 Products

Product Documents for ATF2 [p Thr71] Antibody (103C411.2) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for ATF2 [p Thr71] Antibody (103C411.2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.