Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATF6 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ATF6. The immunogen is located within amino acids 30 - 80 of ATF6. Amino Acid Squence: ELQLEAANETYENNFDN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit ATF6 Antibody - BSA Free (NBP1-77251) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-ATF6 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ATF6 Antibody - BSA Free

ATF6 Antibody - BSA Free

Immunohistochemistry: ATF6 Antibody - BSA Free [NBP1-77251] -

Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ATF6 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
ATF6 Antibody - BSA Free

Western Blot: ATF6 Antibody - BSA Free [NBP1-77251] -

Loading: 15 ug of rat kidney lysate Antibodies: ATF6, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
ATF6 Antibody - BSA Free

Western Blot: ATF6 Antibody - BSA Free [NBP1-77251] -

Loading: 15 ug of mouse tissue lysate Antibodies: ATF6, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
ATF6 Antibody - BSA Free

Immunohistochemistry: ATF6 Antibody - BSA Free [NBP1-77251] -

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ATF6 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
ATF6 Antibody - BSA Free

Western Blot: ATF6 Antibody - BSA Free [NBP1-77251] -

Loading: 15 ug of human HeLa cell lysate Antibodies ATF6, 0.5 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
ATF6 Antibody - BSA Free

Western Blot: ATF6 Antibody - BSA Free [NBP1-77251] -

Loading: 15 ug of human cell lysate Antibodies ATF6, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.conjugate at 1:10000 dilution.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.conjugate at 1:10000 dilution.
ATF6 Antibody - BSA Free

Immunohistochemistry: ATF6 Antibody - BSA Free [NBP1-77251] -

Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ATF6 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Applications for ATF6 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

5 ug/mL

Western Blot

0.5-1 ug/ml
Application Notes
Use in IHC-P reported in scientific literature (PMID:33603856).

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATF6

Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR. ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes. ATF6 has two isoforms (ATF6alpha and ATF6beta); only ATF6alpha is recognized by this antibody.

Long Name

Activating Transcription Factor 6

Alternate Names

ACHM7, ATF6 alpha

Gene Symbol

ATF6

UniProt

NP_031374 (Human)

Additional ATF6 Products

Product Documents for ATF6 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ATF6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ATF6 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ATF6 Antibody - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

    • Q: May we ask if ATF6 is ATF6 alpha? We are looking for ATF6 alpha antibody for our customer, but found none from Novus. Only ATF6 antibodies. However, after checking http://www.uniprot.org/uniprot/P18850, I found ATF6 and ATF6 alpha seem identical. Would you please help confirm if NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439 are all ATF6 alpha antibodies?
      A: The antibodies NBP1-77251, NBP1-76675 are specific to ATF6A and should not cross-react with ATF6B.

    • Q: We are looking for an antibody specific to ATF6 alpha that will not cross-react with ATF6 beta. Would you please help confirm if any of the following are ATF6 alpha specific antibodies: NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439?
      A: The antibodies NBP1-77251 and NBP1-76675 are specific to ATF6 alpha and should not cross-react with ATF6 beta.
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