ATG12 Antibody (166) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43781

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 166

Format

Azide and BSA Free
View all ATG12 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG12. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (81%), Chimpanzee (82%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATG12 Antibody (166) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: ATG12 Antibody (166) [NBP2-43781]

Immunocytochemistry/ Immunofluorescence: ATG12 Antibody (166) [NBP2-43781]

Immunocytochemistry/Immunofluorescence: ATG12 Antibody (166) [NBP2-43781] - Analysis of Samples: HeLa cells mock (left) and treated with 50uM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG12 protein stained by ATG12 antibody [166]. Red: Phalloidin, a F-actin marker.
Western Blot: ATG12 Antibody (166) [NBP2-43781]

Western Blot: ATG12 Antibody (166) [NBP2-43781]

Western Blot: ATG12 Antibody (166) [NBP2-43781] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [166] dilution: 1:1000
Western Blot: ATG12 Antibody (166) [NBP2-43781]

Western Blot: ATG12 Antibody (166) [NBP2-43781]

Western Blot: ATG12 Antibody (166) [NBP2-43781] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw264.7 whole cell lysate/extract G. 30 ug C2C12 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [166] dilution: 1:1000
ATG12 Antibody (166) - Azide and BSA Free

Western Blot: ATG12 Antibody (166) [NBP2-43781] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody [GT166] (NBP2-43781) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for ATG12 Antibody (166) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
PLA-Assay dependent.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATG12

The ATG12 gene codes a ubiquitin-like protein ATG12 that in isoform 1 is 140 amino acids long at 15 kDA and in isoform 2 is 74 amino acids long at 7 kDA. This protein is critical for autophagy (bulk protein degradation) as it is the human homolog of a yeast protein that participates in autophagy. ATG12 participates in the immune system, apoptosis and autophagy, protein stability, negative regulators of RIG-I/MDA5 signaling, and glucose/energy metabolism. It is known to interact with various genes such as HIST1H2BF, HIST1H2BE, HIST1H2BI, HIST1H2BG, and HIST1H2BC. ATG12 has been researched regarding its role in leukemia, lung cancer, myositis, retinitis, carcinoma, colorectal cancer, and myelodysplastic syndrome.

Long Name

ATG12 Autophagy Related 12 Homolog

Alternate Names

APG12, FBR93, HAPG12

Gene Symbol

ATG12

Additional ATG12 Products

Product Documents for ATG12 Antibody (166) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ATG12 Antibody (166) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy

Citations for ATG12 Antibody (166) - Azide and BSA Free

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Protocols

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FAQs

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