ATP Citrate Lyase Antibody (5F8D11) - BSA Free

Novus Biologicals | Catalog # NBP2-37429

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5F8D11

Format

BSA Free
View all ATP Citrate Lyase Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human ATP Citrate Lyase (AA: 306-502) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATP Citrate Lyase Antibody (5F8D11) - BSA Free

Western Blot: ATP Citrate Lyase Antibody (5F8D11)BSA Free [NBP2-37429]

Western Blot: ATP Citrate Lyase Antibody (5F8D11)BSA Free [NBP2-37429]

Western Blot: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Western blot analysis using ACLY mouse mAb against HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate.
Immunocytochemistry/ Immunofluorescence: ATP Citrate Lyase Antibody (5F8D11) - BSA Free [NBP2-37429]

Immunocytochemistry/ Immunofluorescence: ATP Citrate Lyase Antibody (5F8D11) - BSA Free [NBP2-37429]

Immunocytochemistry/Immunofluorescence: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Immunofluorescence analysis of HeLa cells using ACLY mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (5F8D11) - BSA Free [NBP2-37429]

Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (5F8D11) - BSA Free [NBP2-37429]

Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Analysis of paraffin-embedded endometrial cancer tissues using ACLY mouse mAb with DAB staining.
Flow Cytometry: ATP Citrate Lyase Antibody (5F8D11) - BSA Free [NBP2-37429]

Flow Cytometry: ATP Citrate Lyase Antibody (5F8D11) - BSA Free [NBP2-37429]

Flow Cytometry: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Flow cytometric analysis of HeLa cells using ACLY mouse mAb (green) and negative control (purple).
Western Blot: ATP Citrate Lyase Antibody (5F8D11)BSA Free [NBP2-37429]

Western Blot: ATP Citrate Lyase Antibody (5F8D11)BSA Free [NBP2-37429]

Western Blot: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Western blot analysis using ACLY mAb against human ACLY recombinant protein. (Expected MW is 46.7 kDa)
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (5F8D11) - BSA Free [NBP2-37429]

Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (5F8D11) - BSA Free [NBP2-37429]

Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Analysis of paraffin-embedded esophageal cancer tissues using ACLY mouse mAb with DAB staining.
ELISA: ATP Citrate Lyase Antibody (5F8D11) - BSA Free [NBP2-37429]

ELISA: ATP Citrate Lyase Antibody (5F8D11) - BSA Free [NBP2-37429]

ELISA: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for ATP Citrate Lyase Antibody (5F8D11) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP Citrate Lyase

ATP citrate lyase (ACLY), also known as ATP citrate synthase, is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACLY and insulin to increase phosphorylation of ACLY (2).

Long Name

ATP-citrate synthase

Alternate Names

ACLY, EC 2.3.3.8

Gene Symbol

ACLY

Additional ATP Citrate Lyase Products

Product Documents for ATP Citrate Lyase Antibody (5F8D11) - BSA Free

Certificate of Analysis

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Product Specific Notices for ATP Citrate Lyase Antibody (5F8D11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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