ATP6V0D2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-31600

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATP6V0D2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: EDRETLYPTFGKLYPEGLRLLAQAEDFDQMKNVADHYGVYKPLFEAVGGSG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATP6V0D2 Antibody - BSA Free

Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600]

Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600]

Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600] - Analysis in human kidney and pancreas tissues. Corresponding ATP6V0D2 RNA-seq data are presented for the same tissues.
Western Blot: ATP6V0D2 Antibody [NBP2-31600]

Western Blot: ATP6V0D2 Antibody [NBP2-31600]

Western Blot: ATP6V0D2 Antibody [NBP2-31600] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Kidney tissue
Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600]

Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600]

Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600] - Staining of human tonsil shows no positivity in germinal center and non-germinal center cells as expected.
Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600]

Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600]

Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600] - Staining of human kidney shows high expression.
Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600]

Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600]

Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600] - Human kidney tissue. Heat mediated antigen retrieval was performed by heating in citrate buffer (pH 6) at 95C for 20 minutes. Image from verified customer review.
Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600]

Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600]

Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600]

Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600]

Immunohistochemistry-Paraffin: ATP6V0D2 Antibody [NBP2-31600] - Staining of human small intestine shows no positivity in glandular cells as expected.

Applications for ATP6V0D2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP2-31600 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP6V0D2

ATP6V0D2 - ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d isoform 2

Alternate Names

ATP6D2, ATP6V0, ATPase, H+ transporting, lysosomal 38kD, V0 subunit d isoform 2, ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d isoform 2, ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2, FLJ38708, Vacuolar proton pump subunit d 2, V-ATPase subunit d 2, VMA6, V-type proton ATPase subunit d 2

Gene Symbol

ATP6V0D2

UniProt

Q8N8Y2 (Human)

Additional ATP6V0D2 Products

Product Documents for ATP6V0D2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ATP6V0D2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ATP6V0D2 Antibody - BSA Free

Customer Reviews for ATP6V0D2 Antibody - BSA Free (1)

5 out of 5
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  • ATP6V0D2 Antibody
    Name: Santhosh Sivajothi
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human kidney tissue
    Species: Human
    Verified Customer | Posted 05/22/2018
    Heat mediated antigen retrieval was performed by heating in citrate buffer (pH6) at 95C for 20 minutes
    ATP6V0D2 Antibody - BSA Free NBP2-31600

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Protocols

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