ATP6V1A Antibody (GT811) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13541

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (98%), Mouse (96%), Porcine (96%), Rat (95%), Rhesus Macaque (99%), Xenopus (90%). Backed by our 100% Guarantee.

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # GT811

Format

Azide and BSA Free
View all ATP6V1A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATP6V1A. The exact sequence is proprietary.

Reactivity Notes

Chicken (82%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Centrifuge briefly prior to opening.

Scientific Data Images for ATP6V1A Antibody (GT811) - Azide and BSA Free

Western Blot: ATP6V1A Antibody (GT811) [NBP3-13541]

Western Blot: ATP6V1A Antibody (GT811) [NBP3-13541]

Western Blot: ATP6V1A Antibody (GT811) [NBP3-13541] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody [GT811] (NBP3-13541) diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: ATP6V1A Antibody (GT811) [NBP3-13541]

Immunocytochemistry/ Immunofluorescence: ATP6V1A Antibody (GT811) [NBP3-13541]

Immunocytochemistry/Immunofluorescence: ATP6V1A Antibody (GT811) [NBP3-13541] - ATP6V1A antibody [GT811] detects ATP6V1A protein at cytoplasm by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATP6V1A protein stained by ATP6V1A antibody [GT811] (NBP3-13541) diluted at 1:200. Blue: Hoechst 33342 staining.
Flow Cytometry: ATP6V1A Antibody (GT811) [NBP3-13541]

Flow Cytometry: ATP6V1A Antibody (GT811) [NBP3-13541]

Flow Cytometry: ATP6V1A Antibody (GT811) [NBP3-13541] - ATP6V1A antibody [GT811] (NBP3-13541) detects ATP6V1A protein by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: ATP6V1A antibody [GT811] (NBP3-13541) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.
ATP6V1A Antibody (GT811)

Immunohistochemistry-Paraffin: ATP6V1A Antibody (GT811) [NBP3-13541] -

Immunohistochemistry-Paraffin: ATP6V1A Antibody (GT811) [NBP3-13541] - ATP6V1A antibody [GT811] detects ATP6V1A protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
ATP6V1A stained by ATP6V1A antibody [GT811] (NBP3-13541) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ATP6V1A Antibody (GT811) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: ATP6V1A Antibody (GT811) [NBP3-13541] -

ATP6V1A antibody [GT811] detects ATP6V1A protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATP6V1A stained by ATP6V1A antibody [GT811] (NBP3-13541) diluted at 1:500.
Blue: Fluoroshield with DAPI.
ATP6V1A Antibody (GT811) - Azide and BSA Free

Western Blot: ATP6V1A Antibody (GT811) [NBP3-13541] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody [GT811] (NBP3-13541) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for ATP6V1A Antibody (GT811) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP6V1A

ATP6V1A encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq]

Long Name

V-type proton ATPase catalytic subunit A

Alternate Names

ATP6A1, ATP6V1A1, EC 7.1.2.2, V-ATPase subunit A, VPP2

Gene Symbol

ATP6V1A

Additional ATP6V1A Products

Product Documents for ATP6V1A Antibody (GT811) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ATP6V1A Antibody (GT811) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ATP6V1A Antibody (GT811) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review ATP6V1A Antibody (GT811) - Azide and BSA Free and earn rewards!

Have you used ATP6V1A Antibody (GT811) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies