ATP6V1H Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32980

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Predicted:

Bovine (98%), Chicken (96%), Porcine (99%), Xenopus (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATP6V1H Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATP6V1H. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (94%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATP6V1H Antibody - BSA Free

Immunohistochemistry-Paraffin: ATP6V1H Antibody [NBP1-32980]

Immunohistochemistry-Paraffin: ATP6V1H Antibody [NBP1-32980]

Immunohistochemistry-Paraffin: ATP6V1H Antibody [NBP1-32980] - Mouse heart. ATP6V1H antibody [N3C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
ATP6V1H Antibody

Immunohistochemistry-Paraffin: ATP6V1H Antibody [NBP1-32980] -

Immunohistochemistry-Paraffin: ATP6V1H Antibody [NBP1-32980] - Mouse kidney.ATP6V1H stained by ATP6V1H antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ATP6V1H Antibody

ICC/IF-ATP6V1H Antibody-NBP1-32980-

ICC/IF-ATP6V1H Antibody-NBP1-32980- DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATP6V1H stained by ATP6V1H antibody [N3C3] diluted at 1:500.Red: Tau, a marker, stained by Tau antibody [287] diluted at 1:500.
ATP6V1H Antibody

IHC-Wm-ATP6V1H Antibody-NBP1-32980-

IHC-Wm-ATP6V1H Antibody-NBP1-32980-2 days-post-fertilization zebrafish embryo.ATP6V1H antibody [N3C3] dilution: 1:50.
ATP6V1H Antibody

Western Blot: ATP6V1H Antibody [NBP1-32980] -

Western Blot: ATP6V1H Antibody [NBP1-32980] - Zebrafish tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1H antibody [N3C3] (NBP1-32980) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
ATP6V1H Antibody

Western Blot: ATP6V1H Antibody [NBP1-32980] -

Western Blot: ATP6V1H Antibody [NBP1-32980] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1H antibody [N3C3] (NBP1-32980) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ATP6V1H Antibody

Western Blot: ATP6V1H Antibody [NBP1-32980] -

Western Blot: ATP6V1H Antibody [NBP1-32980] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1H antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
ATP6V1H Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ATP6V1H Antibody [NBP1-32980] -

ATP6V1H antibody [N3C3] detects ATP6V1H protein by immunofluorescent analysis.
Sample: T-47D cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATP6V1H stained by ATP6V1H antibody [N3C3] (NBP1-32980) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.

Applications for ATP6V1H Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry Whole-Mount

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATP6V1H

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the regulatory H subunit of the V1 domain which is required for catalysis of ATP but not the assembly of V-ATPase. Three alternatively spliced transcript variants encode two isoforms of the H subunit.

Alternate Names

ATPase, H+ transporting, lysosomal 50/57kD, V1 subunit H, ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H, CGI-11, MSTP042, NBP1, Nef-binding protein 1, Protein VMA13 homolog, SFD, SFDalpha, SFDbeta, vacuolar ATP synthase subunit H, vacuolar ATPase subunit H, vacuolar proton pump H subunit, Vacuolar proton pump subunit H, Vacuolar proton pump subunit SFD, V-ATPase 50/57 kDa subunits, V-ATPase H subunit, V-ATPase subunit H, VMA13, V-type proton ATPase subunit H

Gene Symbol

ATP6V1H

Additional ATP6V1H Products

Product Documents for ATP6V1H Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ATP6V1H Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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