ATR [p Ser428] Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-05447

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATR Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S428 of human ATR (NP_001175.2). GISPK

Modification

p Ser428

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

301 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit ATR [p Ser428] Antibody - BSA Free (NBP3-05447) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ATR [p Ser428] Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ATR [p Ser428] Antibody - Azide and BSA Free [NBP3-05447]

Immunocytochemistry/ Immunofluorescence: ATR [p Ser428] Antibody - Azide and BSA Free [NBP3-05447]

Immunocytochemistry/Immunofluorescence: ATR [p Ser428] Antibody [NBP3-05447] - Immunofluorescence analysis of U2OS cells using ATR antibody (NBP3-05447) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody - Azide and BSA Free [NBP3-05447]

Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody - Azide and BSA Free [NBP3-05447]

Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Rat testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody - Azide and BSA Free [NBP3-05447]

Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody - Azide and BSA Free [NBP3-05447]

Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Human testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody - Azide and BSA Free [NBP3-05447]

Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody - Azide and BSA Free [NBP3-05447]

Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Mouse testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ATR [p Ser428] Antibody

Western Blot: ATR [p Ser428] Antibody [NBP3-05447] -

Western Blot: ATR [p Ser428] Antibody [NBP3-05447] - Analysis of 293T, using Phospho-ATR-S428 antibody at 1:400 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
ATR [p Ser428] Antibody

Western Blot: ATR [p Ser428] Antibody [NBP3-05447] -

Western Blot: ATR [p Ser428] Antibody [NBP3-05447] - Analysis of NIH/3T3, using Phospho-ATR-S428 antibody at 1:400 dilution.NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37 for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for ATR [p Ser428] Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATR

Ataxia Telangiectasia Mutated (ATM) and Rad3- related (ATR) protein is a member of the phosphatidyl-inositol 3-kinase (PI 3-kinase) like family of protein kinases. ATR has a predicted molecular mass of approximately 301 kDa, and it has been shown to be involved in cellular responses to DNA damage. Specifically, it plays a role in checkpoint activation in response to either the presence of stalled replication forks, or by the presence of lesions caused by UV light. Inactivation of ATR is an embryonic lethal event in mice.

Long Name

Ataxia Telangiectasia and Rad 3-related

Alternate Names

FRP1, MEC1, SCKL1

Gene Symbol

ATR

Additional ATR Products

Product Documents for ATR [p Ser428] Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ATR [p Ser428] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

p53 Pathway

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Protocols

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FAQs

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