Atrial Natriuretic Peptide/ANP Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35642

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Atrial Natriuretic Peptide/ANP Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 26-151 of human Atrial Natriuretic Peptide/ANP (NP_006154.1).

Sequence:
LTWQEIMSITELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSIHPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQEDPESDSGLSLN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Atrial Natriuretic Peptide/ANP Antibody - BSA Free

Atrial Natriuretic Peptide/ANP Antibody

Western Blot: Atrial Natriuretic Peptide/ANP Antibody [NBP3-35642] -

Western Blot: Atrial Natriuretic Peptide/ANP Antibody [NBP3-35642] - Western blot analysis of various lysates using Atrial Natriuretic Peptide/ANP Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Atrial Natriuretic Peptide/ANP Antibody

Immunocytochemistry/ Immunofluorescence: Atrial Natriuretic Peptide/ANP Antibody [NBP3-35642] -

Immunocytochemistry/ Immunofluorescence: Atrial Natriuretic Peptide/ANP Antibody [NBP3-35642] - Immunofluorescence analysis of paraffin-embedded mouse heart using Atrial Natriuretic Peptide/ANP Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Atrial Natriuretic Peptide/ANP Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Atrial Natriuretic Peptide/ANP

Atrial natriuretic factor (ANP) is a potent vasoactive substance which is thought to play a key role in cardiovascular homeostasis and has a cGMP stimulating activity. There are two different prepronatriodilatin alleles. One codes for 2 Arginine residues at the C terminus that are cleaved to form the mature peptide, while the other ends in a termination codon immediately after the last codon of the mature peptide. ANP belongs to the natriuretic peptide family.

Alternate Names

ANF, ANP, Cardionatrin, NPPA, PND

Gene Symbol

NPPA

Additional Atrial Natriuretic Peptide/ANP Products

Product Documents for Atrial Natriuretic Peptide/ANP Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Atrial Natriuretic Peptide/ANP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cardiac Stem Cell Markers

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Protocols

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