AU5 Epitope Tag Antibody - BSA Free
Novus Biologicals | Catalog # NB600-461
![Western Blot: AU5 Epitope Tag AntibodyBSA Free [NB600-461]](https://resources.rndsystems.com/images/products/AU5-Epitope-Tag-Antibody-Western-Blot-NB600-461-img0002.jpg "Western Blot: AU5 Epitope Tag AntibodyBSA Free [NB600-461]")
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Species Reactivity
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Scientific Data Images for AU5 Epitope Tag Antibody - BSA Free
Western Blot: AU5 Epitope Tag AntibodyBSA Free [NB600-461]
Western Blot: AU5 Epitope Tag Antibody [NB600-461] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).Applications for AU5 Epitope Tag Antibody - BSA Free
ELISA
Immunocytochemistry/ Immunofluorescence
Immunoprecipitation
Western Blot
Formulation, Preparation, and Storage
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Background: AU5 Epitope Tag
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Product Documents for AU5 Epitope Tag Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AU5 Epitope Tag Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for AU5 Epitope Tag Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for AU5 Epitope Tag Antibody - BSA Free
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Q: Do you have any kinetic data on the antibody affinity (i.e. Kd, Kon, Koff)?
A: The determination of Kd, on-rates, and off-rates are only possible with pure protein preparations where concentration and amounts are known and controlled. This is possible when measuring monoclonal antibody affinity to its target protein. However, polyclonal populations, such as this product, are not uniform and contain a mixture. Unfortunately, measuring the Kd of such a mixture is not possible.