Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Aurora B Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acids 1-19 of human Aurora B with C-terminal added cysteine conjugated to KLH. Uniprot accession no. Q96GD4

Marker

Mitosis Marker

Specificity

Aurora B

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Aurora B Antibody - BSA Free (NB100-294) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-Aurora B Antibody: Cited in 12 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Aurora B Antibody - BSA Free

Western Blot: Aurora B AntibodyBSA Free [NB100-294]

Western Blot: Aurora B AntibodyBSA Free [NB100-294]

Western Blot: Aurora B Antibody - BSA Free [NB100-294] - Whole extract of PC-12 cells were separated on SDS-PAGE and probed with Anti-Aurora B antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:1000 antibody2. 1:2000 antibody3. Negative Control
Immunocytochemistry/ Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294]

Immunocytochemistry/ Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294]

Immunocytochemistry/Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294] - HeLa human cervix epitheloid carcinoma cells were fixed and permeabilized with 4% paraformaldehyde followed by cold methanol. Fixed cells were stained with 1:50 Anti-Aurora B antibody produced in rabbit. The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in goat.

Applications for Aurora B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

50-60 ug

Western Blot

1:1000-1:2000
Application Notes
By Western blot, it detects a band of approximately 41 kDa. Detection of the Aurora B band by immunoblotting is specifically inhibited with the immunizing peptide.

Reviewed Applications

Read 5 reviews rated 4.8 using NB100-294 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

12.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aurora B

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A localizes to centrosomes.

Alternate Names

Aik2, AIM-1, AIM1, ARK2, AURKB, IPL1, STK12, STK5

Entrez Gene IDs

9212 (Human); 20877 (Mouse); 114592 (Rat)

Gene Symbol

AURKB

Additional Aurora B Products

Product Documents for Aurora B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Aurora B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Epigenetics
Microtubules

Citations for Aurora B Antibody - BSA Free

Customer Reviews for Aurora B Antibody - BSA Free (5)

4.8 out of 5
5 Customer Ratings
5 Stars
80%
4 Stars
20%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: RPE1 cells
    Species: Human
    Verified Customer | Posted 04/21/2016
    Prometaphase Aurora B
    Aurora B Antibody - BSA Free NB100-294
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: 293T cell line
    Species: Human
    Verified Customer | Posted 10/02/2015
    Aurora B on 293T
    Aurora B Antibody - BSA Free NB100-294
  • Name: Andrew Cosgrove
    Application: Immunocytochemistry
    Sample Tested: RPE-1 cells
    Species: Human
    Verified Customer | Posted 11/06/2013
    Aurora B Antibody - BSA Free NB100-294
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested:
    Species: Human
    Verified Customer | Posted 10/04/2013
  • Name: Duane Compton
    Application: Immunofluorescence
    Sample Tested: RPE-1
    Species: Human
    Verified Customer | Posted 05/02/2012

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Protocols

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FAQs

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