Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Aurora B Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acids 1-19 of human Aurora B with C-terminal added cysteine conjugated to KLH. Uniprot accession no. Q96GD4

Marker

Mitosis Marker

Specificity

Aurora B

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aurora B Antibody - BSA Free

Western Blot: Aurora B AntibodyBSA Free [NB100-294]

Western Blot: Aurora B AntibodyBSA Free [NB100-294]

Western Blot: Aurora B Antibody - BSA Free [NB100-294] - Whole extract of PC-12 cells were separated on SDS-PAGE and probed with Anti-Aurora B antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:1000 antibody2. 1:2000 antibody3. Negative Control
Immunocytochemistry/ Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294]

Immunocytochemistry/ Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294]

Immunocytochemistry/Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294] - HeLa human cervix epitheloid carcinoma cells were fixed and permeabilized with 4% paraformaldehyde followed by cold methanol. Fixed cells were stained with 1:50 Anti-Aurora B antibody produced in rabbit. The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in goat.
Aurora B Antibody - BSA Free

Western Blot: Aurora B Antibody - BSA Free [NB100-294] -

AURKB mediates EGF associated BRAF/MEK/ERKs and PI3-K/AKT pathways. (A, B) Knocking down of AURKB suppressed the level of EGF in A375 and A375R tumor from xenograft mice compared with shcontrol. The asterisks indicate a significant difference compared with the shcontrol group (*, p < 0.05; ** p < 0.01). (C) Knocking down of AURKB increased levels of c-PARP, c-caspase 3, and decreased of Bcl-2 level of A375 and A375R cell lines. (D, E) Western blot analysis of activation of BRAF/MEK/ERKs and PI3-K/AKT by knocking down of AURKB in A375 and A375R cell lines. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32863956), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Aurora B Antibody - BSA Free

Western Blot: Aurora B Antibody - BSA Free [NB100-294] -

Database analysis and AURKB expression level in melanoma. (A) Volcano plot of gene expression analysis in GSE 4587. The X-axis indicates the fold change between normal skin or nevi and melanoma samples, and the Y-axis indicates on a log10 scale the p-values obtained from a supervised logistic regression analysis testing the association of gene expression between normal skin or nevi/melanoma. The horizontal dotted lines mark the significance cutoffs (i.e., fold change more than 4 times or less than 0.25, and p-value less than 0.001). (B) Heat map of analysis of the expression of these selected genes in GSE 4587. AURKB has a higher expression level in melanoma compared with non-melanoma (normal skin and nevi). (C) Pathway enrichment analysis of differential gene analysis. The top 2 pathways were selected, fold change more than 4 times, p-value less than 0.0001. (D) The Venn diagram lists the common genes in both pathways. (E) Melanoma patients with high expression of AURKB show a significantly lower overall survival rate. (F)AURKB is highly expressed in melanoma compared with normal skin as determined by GEPIA. (G) AURKB is significantly overexpressed in melanoma tissue compared with normal skin as shown in a tissue array analysis; the scale bar = 100 um. (H) AURKB is overexpressed in drug-resistant melanoma cell lines (A375R, M238R, and M249R) compared with drug-sensitive melanoma cell lines (A375, M238 and M249). Statistical significance was determined by Student's t-test and the asterisks indicate a significant change compared with the control group (*, p < 0.05; ***, p < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32863956), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Aurora B Antibody - BSA Free

Western Blot: Aurora B Antibody - BSA Free [NB100-294] -

AURKB mediates EGF associated BRAF/MEK/ERKs and PI3-K/AKT pathways. (A, B) Knocking down of AURKB suppressed the level of EGF in A375 and A375R tumor from xenograft mice compared with shcontrol. The asterisks indicate a significant difference compared with the shcontrol group (*, p < 0.05; ** p < 0.01). (C) Knocking down of AURKB increased levels of c-PARP, c-caspase 3, and decreased of Bcl-2 level of A375 and A375R cell lines. (D, E) Western blot analysis of activation of BRAF/MEK/ERKs and PI3-K/AKT by knocking down of AURKB in A375 and A375R cell lines. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32863956), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Aurora B Antibody - BSA Free

Western Blot: Aurora B Antibody - BSA Free [NB100-294] -

HI-511 binds to and effectively suppresses the activation of AURKB and BRAF V600E. (A) The structure of HI-511. (B) Computer docking model of HI-511 binding with AURKB. (C) Computer docking model of HI-511 binding with BRAF V600E. (D) HI-511 inhibits AURKB in vitro. Active kinase AURKB and HI-511 (0, 1, 5 and10 uM) or APIO-EE-9 (5 uM) were mixed with the substrate histone H3. The relative amounts of phosphorylated substrate were visualized by Western blot. (E) HI-511 inhibits BRAF V600E in vitro. Active kinase BRAF V600E and HI-511 (0, 1, 5, 10 uM) or vemurafenib (5 uM) were mixed with the substrate phosphatidylinositol. The relative amounts of phosphorylated substrate were visualized by Western blot. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32863956), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Aurora B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

50-60 ug

Western Blot

1:1000-1:2000
Application Notes
By Western blot, it detects a band of approximately 41 kDa. Detection of the Aurora B band by immunoblotting is specifically inhibited with the immunizing peptide.

Reviewed Applications

Read 5 reviews rated 4.8 using NB100-294 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

12.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aurora B

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A localizes to centrosomes.

Alternate Names

Aik2, AIM-1, AIM1, ARK2, AURKB, IPL1, STK12, STK5

Entrez Gene IDs

9212 (Human); 20877 (Mouse); 114592 (Rat)

Gene Symbol

AURKB

Additional Aurora B Products

Product Documents for Aurora B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Aurora B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Epigenetics
Microtubules

Citations for Aurora B Antibody - BSA Free

Customer Reviews for Aurora B Antibody - BSA Free (5)

4.8 out of 5
5 Customer Ratings
5 Stars
80%
4 Stars
20%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: RPE1 cells
    Species: Human
    Verified Customer | Posted 04/21/2016
    Prometaphase Aurora B
    Aurora B Antibody - BSA Free NB100-294
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: 293T cell line
    Species: Human
    Verified Customer | Posted 10/02/2015
    Aurora B on 293T
    Aurora B Antibody - BSA Free NB100-294
  • Name: Andrew Cosgrove
    Application: Immunocytochemistry
    Sample Tested: RPE-1 cells
    Species: Human
    Verified Customer | Posted 11/06/2013
    Aurora B Antibody - BSA Free NB100-294
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested:
    Species: Human
    Verified Customer | Posted 10/04/2013
  • Name: Duane Compton
    Application: Immunofluorescence
    Sample Tested: RPE-1
    Species: Human
    Verified Customer | Posted 05/02/2012

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Protocols

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