Autoimmune Regulator/AIRE Antibody

Novus Biologicals | Catalog # NB100-40799

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Porcine

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Autoimmune Regulator/AIRE Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KDVDLSQPRKGRKP corresponding to internal region according to NP_000374.1.

Specificity

This antibody is expected to recognise isoform 1 (NP_000374.1) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Autoimmune Regulator/AIRE Antibody

Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799]

Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799]

Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799] - HEK293 cell lysates at 0.05ug/mL. Untransfected (lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.
Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-40799]

Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-40799]

Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-40799] - Staining of Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799]

Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799]

Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799] - Analysis of HEK293 cell lysates. Untransfected (Lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.

Applications for Autoimmune Regulator/AIRE Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 ug/mL

Immunohistochemistry-Paraffin

2.5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 10 ug/mL
Application Notes
WB: Approx. 60 kDa band in transfected HEK293 lysates (calculated MW of 57.7 kDa band according to NP_000374.1). IHC-P: Human thymus shows nuclear staining in select thymocytes. IHC usage on fetal porcine thymus is reported in scientific literature (PMID: 24770647).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Autoimmune Regulator/AIRE

This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

Alternate Names

AIRE, APECED, APS1, APSI, PGA1

Entrez Gene IDs

326 (Human)

Gene Symbol

AIRE

UniProt

NP_000374.1 (Human)

Additional Autoimmune Regulator/AIRE Products

Product Documents for Autoimmune Regulator/AIRE Antibody

Certificate of Analysis

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Product Specific Notices for Autoimmune Regulator/AIRE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Autoimmune Regulator/AIRE Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Autoimmune Regulator/AIRE Antibody

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  • Q: I am looking into a positive control for Western blotting. We are trying to detect the AIRE protein and are not sure whether the antibody we have is picking up the protein. Are you able to tell me whether the human thymus whole tissue lysate (NB820-59456) has been used to detect for AIRE expression by Western blotting?

    A:

    I am not currently aware of any references to the use of our human thymus whole tissue lysate being used to detect AIRE by Western blotting. On looking through the Western blot images for all seven of our AIRE antibodies, I see that these have been generated using lysates from RT-4 cells (bladder), U-251 MG cells (brain), 293 cells (human embryonic kidney), human spleen lysate, liver lysate and tonsil lysate. Additionally, you may find the Human Protein Atlas link to AIRE useful. At this site you can find protein expression profiles based on immunohistochemistry for a large number of human tissues, cancers and cell lines: The Human Protein Atlas.

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