Axin-1 Antibody

Name: Axin-1 Antibody
Brand: Novus Biologicals
SKU: NB100-1011
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-1011

Submit a review

Citations (3)

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Axin-1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-FEEKIIGKVEKVD corresponding to C-Terminus according to NP_851393.1.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Specificity

This antibody is expected to recognise both reported isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Axin-1 Antibody

Immunohistochemistry: Axin-1 Antibody [NB100-1011]

Immunohistochemistry: Axin-1 Antibody [NB100-1011] - (3.8ug/ml) staining of paraffin embedded Human Adrenal Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Axin-1 Antibody

Application

Recommended Usage

Immunohistochemistry

3 - 5 ug/ml

Immunohistochemistry-Paraffin

3 - 5 ug/ml

Peptide ELISA

Detection limit 1:128000

Application Notes

WB: Preliminary experiments gave bands at approx. 150 kDa band and 22-24 kDa band in human brain (hippocampus) lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.6 kDa band according to NP_003493.1 and 91.7 kDa band according to NP_851393.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P: Human adrenal gland shows membranous and fibrous staniing in and around secretory cells of the zona fasciculata in the cortex.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Axin-1

Negative regulator of Wnt signaling. Also associated with cancer.

Long Name

Axis Inhibitor 1

Alternate Names

Axin1, MGC52315

Entrez Gene IDs

8312 (Human); 12005 (Mouse); 79257 (Rat)

Gene Symbol

AXIN1

UniProt

NP_003493 (Human)

Additional Axin-1 Products

Product Documents for Axin-1 Antibody

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Axin-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Wnt Family

Citations for Axin-1 Antibody

Customer Reviews for Axin-1 Antibody

There are currently no reviews for this product. Be the first to review Axin-1 Antibody and earn rewards!

Have you used Axin-1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image