Axin-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-25292

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein Axin-1 using the following amino acid sequence: AWHHFPPRCVDMGCAGLRDAHEENPESILDEHVQRVLRTPGRQSPGPGHRSPDSGHVAKMPVALGGAASGHGKHVPKS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Axin-1 Antibody - BSA Free

Axin-1 Antibody Western Blot: Axin-1 Antibody [NBP3-25292]

Western Blot: Axin-1 Antibody [NBP3-25292]

Lane 1: Marker [kDa] 250,130,95,72,55,36,28,17,10
Lane 2: RT-4
Lane 3: U-251MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
Axin-1 Antibody Immunocytochemistry/Immunofluorescence: Axin-1 Antibody [NBP3-25292]

Immunocytochemistry/Immunofluorescence: Axin-1 Antibody [NBP3-25292]

Staining of human cell line SiHa shows localization to nucleoli.

Applications for Axin-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 µg/ml

Western Blot

0.04-0.4 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Axin-1

Negative regulator of Wnt signaling. Also associated with cancer.

Long Name

Axis Inhibitor 1

Alternate Names

Axin1, MGC52315

Gene Symbol

AXIN1

Additional Axin-1 Products

Product Documents for Axin-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Axin-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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