AZ1 Antibody

Name: AZ1 Antibody
Brand: Novus Biologicals
SKU: NB100-68227
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-68227

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all AZ1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1033 and 1083 of human centrosomal protein 131 kDa (5-azacytidine induced 1) using the numbering given in entry EAW89632.1 (GeneID 22994).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AZ1 Antibody

Western Blot: AZ1 Antibody [NB100-68227]

Western Blot: AZ1 Antibody [NB100-68227] - Detection of Human CEP131/AZ1 on HeLa whole cell lysate using NB100-68227. CEP131/AZ1 was also immunoprecipitated by rabbit anti-CEP131/AZ1 antibodies NB100-68225 and NB100-68226.

Immunocytochemistry/ Immunofluorescence: AZ1 Antibody [NB100-68227]

Immunocytochemistry/Immunofluorescence: AZ1 Antibody [NB100-68227] - NBF-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:200.

Applications for AZ1 Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Application Notes

ICC/IF Formaldehyde fixation with Triton-X 100 permeabilization is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: AZ1

CEP131/AZ1 was originally identified as a transcript that was increased in cells exposed to the demethylating agent, azacytidine. It was found to localize to the pre-acrosome region of spermatids and therefore thought to play a role in spermatogenesis. CEP131/AZ1 has also been identified as a component of the centrosome.

Alternate Names

5-azacytidine induced 1, 5-azacytidine-induced protein 1, AZ1, centrosomal protein 131 kDa, centrosomal protein of 131 kDa, Cep131, KIAA1118, pre-acrosome localization protein 1, ZA1

Entrez Gene IDs

22994 (Human)

Gene Symbol

CEP131

UniProt

EAW89632.1 (Human)

Additional AZ1 Products

Product Documents for AZ1 Antibody

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Certificate of Analysis

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Product Specific Notices for AZ1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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